Protein Info for BWI76_RS27135 in Klebsiella michiganensis M5al

Annotation: selenocysteine-specific translation factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 613 TIGR00475: selenocysteine-specific translation elongation factor" amino acids 1 to 608 (608 residues), 780.5 bits, see alignment E=5.9e-239 PF00009: GTP_EFTU" amino acids 2 to 163 (162 residues), 116 bits, see alignment E=6.4e-37 PF01926: MMR_HSR1" amino acids 3 to 109 (107 residues), 25 bits, see alignment E=7e-09 PF03144: GTP_EFTU_D2" amino acids 189 to 256 (68 residues), 36.4 bits, see alignment E=2.3e-12 PF25461: Beta-barrel_SelB" amino acids 266 to 344 (79 residues), 120.5 bits, see alignment E=1.2e-38 PF21458: WHD_1st_3rd_SelB" amino acids 350 to 398 (49 residues), 67.5 bits, see alignment 2.7e-22 PF09106: WHD_2nd_SelB" amino acids 425 to 481 (57 residues), 55.3 bits, see alignment 2.3e-18 PF21214: WHD_2nd_SelB_bact" amino acids 488 to 546 (59 residues), 106.3 bits, see alignment 2e-34 PF09107: WHD_3rd_SelB" amino acids 566 to 606 (41 residues), 58.6 bits, see alignment 1.4e-19

Best Hits

Swiss-Prot: 80% identical to SELB_ECOLI: Selenocysteine-specific elongation factor (selB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 88% identity to eae:EAE_06175)

MetaCyc: 80% identical to selenocysteyl-tRNA-specific translation elongation factor (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Selenocysteine-specific translation elongation factor" in subsystem Selenocysteine metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BB62 at UniProt or InterPro

Protein Sequence (613 amino acids)

>BWI76_RS27135 selenocysteine-specific translation factor (Klebsiella michiganensis M5al)
MIIATAGHVDHGKTTLLQAITGINADRLPEEKARGMTIDLGYAYWPQPDGRVLGFIDVPG
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLTGKPTLTVALTKADRVETA
RVEEVKAEAEAALAEFGFTGATLFTVAATEGLGIAGLRDHLLQLSARAHPQHQRFRLAID
RAFTVKGAGLVVTGTALSGEVNVGDTLWLTGVDKPMRVRGLHAQNQPVAKAWAGQRIALN
IVGDAQKEDLNRGDWLLAVPPPEASERVIVELQCHTPLSQWQPLHIHHAASHITGRVSLL
EGALAELVLDTPLRLADNDRLVLRDISARLTLAGARVVTLNPPRRGKRKPEYLQWLHALA
AAQGDDVQALDIHLQRDAVRLDDFAWARQLSDEGLKALINRPDYLQAGNSLLSAPLAARW
QRKLLDALARYHDQHRDEPGPGRERLRRIALPMEDEALVLLLIEQMRESGTVHSHHGWLH
LPEHKAGFTDEQQAIWQRVVGLFADEPWWVRDLAREANVEESLMRAVLRQAAQQGMITAI
VKDRYYRNDRIVAFAKMIRELDLERGSTCAADFRDTLNVGRKLAIQILEYFDRIGFTRRR
GNDHILRDKALFL