Protein Info for BWI76_RS27065 in Klebsiella michiganensis M5al
Annotation: valine--pyruvate transaminase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 91% identical to AVTA_ECOLI: Valine--pyruvate aminotransferase (avtA) from Escherichia coli (strain K12)
KEGG orthology group: K00835, valine--pyruvate aminotransferase [EC: 2.6.1.66] (inferred from 95% identity to kpe:KPK_0169)MetaCyc: 91% identical to valine--pyruvate aminotransferase (Escherichia coli K-12 substr. MG1655)
Valine--pyruvate transaminase. [EC: 2.6.1.66]; 2.6.1.- [EC: 2.6.1.66]
Predicted SEED Role
"Valine--pyruvate aminotransferase (EC 2.6.1.66)" in subsystem Alanine biosynthesis or Branched-Chain Amino Acid Biosynthesis or Pyruvate Alanine Serine Interconversions (EC 2.6.1.66)
MetaCyc Pathways
- superpathway of L-alanine biosynthesis (4/4 steps found)
- L-alanine biosynthesis I (2/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.66
Use Curated BLAST to search for 2.6.1.66
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285BB93 at UniProt or InterPro
Protein Sequence (419 amino acids)
>BWI76_RS27065 valine--pyruvate transaminase (Klebsiella michiganensis M5al) MTFSLFGDKFTRHSGITRLMEDLNDGLRTPGAIMLGGGNPAQIPEMNDYFHSLLADMLNS GKALDALCNYDGPQGKSELLTLLAQMLRDELGWEIEPQNIALTNGSQSAFFYLFNLFAGR RADGTTRKVLFPLTPEYIGYADSGLEEDLFVATRPNIELLPEGQFKYHVDFEHLQVTDET GMICVSRPTNPTGNVITDDELIKLDALANQHGIPLVIDNAYGVPFPGIIFSDARPLWNPN IVLCMSLSKLGLPGSRCGIIIANEKIITAISNMNGIISLAPGGMGPAMMCEMIKRNDLLR LSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHKPEGAIFLWLWFKDLPISTELLYQRLK KRGVLMVPGDYFFPGLDKPWPHTHQCMRMNYVPDPEKIEAGVRILAEEVERAWREQDPS