Protein Info for BWI76_RS27060 in Klebsiella michiganensis M5al

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 677 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF00128: Alpha-amylase" amino acids 227 to 370 (144 residues), 101 bits, see alignment E=5.3e-33 amino acids 441 to 595 (155 residues), 35.7 bits, see alignment E=4e-13

Best Hits

Swiss-Prot: 78% identical to AMY1_ECOLI: Periplasmic alpha-amylase (malS) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 86% identity to eae:EAE_06155)

MetaCyc: 78% identical to alpha-amylase (Escherichia coli K-12 substr. MG1655)
Alpha-amylase. [EC: 3.2.1.1]

Predicted SEED Role

"Periplasmic alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.1

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BBN3 at UniProt or InterPro

Protein Sequence (677 amino acids)

>BWI76_RS27060 alpha-amylase (Klebsiella michiganensis M5al)
MKLAALASLLVPGMAFAAWTTADFPAFTEEGTGRFISQNEVAKGTRPLRITFDQQCWQPA
GAIKLNQMLSMEPCRDPAPQWRVFRDGRYTLEVDTRSGTPTLMVSLEEKQTSTAAAPQIK
QCPKWDGKPLTIDVSKSFAEGSQVRDFYSGNIATVSGGKITLQPAFNSNGLLLLERADTE
AAAPFNWHNATVYFVLTDRFVNGNPANDNSYGRHKDGMQEIGTFHGGDLQGLTSKLDYLQ
QLGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYIQDWTKLDANMGTEDDLRRLVDE
AHKRGIRILFDVVMNHTGYATLADMQEFQFGSLYIQGDELKKTLGERWTDWKPRAGQSWH
SFNDYINFSDKAGWEKWWGKKWIRTDIGDYDNPGFDDLTMSLAFLPDLKTESTEPSGLPN
FYRHKPDTAAKAIPGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVEQAAWLQLKTQATE
ALAEWKKANPDKALDNAPFWMTGEAWGHGVMQSDYYRHGFDAMLNFDYQDQAAKATSCLA
NIDLTWQHMADKLQDFNVLSYLSSHDTSLFREGGSTAAELLLLAPGAVQIFYGDESSRPF
GPTGSDPLQGTRSDMNWQDVSGKAARSVTHWEKLGQFRARHPAIGMGKQTTLSMAKGYGF
IRENGDDKVMVVWAGQQ