Protein Info for BWI76_RS27025 in Klebsiella michiganensis M5al

Updated annotation (from data): Xylose isomerase (EC 5.3.1.5)
Rationale: Specifically important for utilizing D-Xylose. Automated validation from mutant phenotype: the predicted function (XYLISOM-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: xylose isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02630: xylose isomerase
  • amino acids 4 to 437 (434 residues), 728.2 bits, E=1.7e-223
• PF27526: XylA2_C
  • amino acids 383 to 437 (55 residues), 78.2 bits, 4.7e-26

Best Hits

Swiss-Prot: 92% identical to XYLA_SALA4: Xylose isomerase (xylA) from Salmonella agona (strain SL483)

KEGG orthology group: None (inferred from 96% identity to eae:EAE_06120)

MetaCyc: 91% identical to xylose isomerase (Escherichia coli K-12 substr. MG1655)
Xylose isomerase. [EC: 5.3.1.5]

Predicted SEED Role

"Xylose isomerase (EC 5.3.1.5)" in subsystem Xylose utilization (EC 5.3.1.5)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (17/17 steps found)
• D-xylose degradation I (2/2 steps found)
• superpathway of pentose and pentitol degradation (21/42 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Pentose and glucuronate interconversions

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.5

Sequence Analysis Tools

See A0A285BBD4 at UniProt or InterPro

Protein Sequence (440 amino acids)

>BWI76_RS27025 Xylose isomerase (EC 5.3.1.5) (Klebsiella michiganensis M5al)
MQTYFDQLDRVRYEGPKSANPLAFRHYNPDELVLGKRMEDHLRFAACYWHTFCWNGADMF
GVGSFNRPWQQPGEAMEMAKRKADVAFEFFHKLNVPYYCFHDVDVSPEGASLKEYANNFA
QMVDVLAEKQQQSGVKLLWGTANCFTNPRYGAGAATNPDPEVFSWAATQVVTAMDATHKL
GGENYVLWGGREGYETLLNTDLRQEREQIGRFMQLVVEHKHKIGFQGTLLIEPKPQEPTK
HQYDYDASTVYGFLKQFGLEKEIKLNIEANHATLAGHTFHHEIATAIALGLFGSVDANRG
DPQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAM
DTMALALKVAARMIEGGELDKRVAKRYAGWNGELGQQILKGQMNLADIAQYATQHNLAPQ
HQSGHQELLENVVNRYLFDR