Protein Info for BWI76_RS26565 in Klebsiella michiganensis M5al

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF01134: GIDA" amino acids 5 to 32 (28 residues), 21.2 bits, see alignment (E = 7.9e-08) PF12831: FAD_oxidored" amino acids 5 to 157 (153 residues), 31.4 bits, see alignment E=7.4e-11 PF03486: HI0933_like" amino acids 5 to 392 (388 residues), 325 bits, see alignment E=1.6e-100 PF07992: Pyr_redox_2" amino acids 5 to 162 (158 residues), 38.1 bits, see alignment E=6.8e-13 PF00890: FAD_binding_2" amino acids 5 to 48 (44 residues), 30.8 bits, see alignment 1e-10 TIGR00275: flavoprotein, HI0933 family" amino acids 7 to 392 (386 residues), 423.9 bits, see alignment E=2.8e-131 PF13450: NAD_binding_8" amino acids 8 to 39 (32 residues), 26 bits, see alignment (E = 5e-09) PF01593: Amino_oxidase" amino acids 101 to 160 (60 residues), 20.1 bits, see alignment E=1.9e-07 PF22780: HI0933_like_1st" amino acids 188 to 339 (152 residues), 167.6 bits, see alignment E=1.2e-52

Best Hits

Swiss-Prot: 85% identical to YHIN_ECOLI: Uncharacterized protein YhiN (yhiN) from Escherichia coli (strain K12)

KEGG orthology group: K07007, (no description) (inferred from 92% identity to kpe:KPK_0250)

Predicted SEED Role

"NAD(FAD)-utilizing dehydrogenases"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BB75 at UniProt or InterPro

Protein Sequence (398 amino acids)

>BWI76_RS26565 membrane protein (Klebsiella michiganensis M5al)
MERFDAIVIGAGAAGMFCAAQAGQAGCRVLLLDNGKKPGRKILMSGGGRCNFTNMYIEPA
AYLSQNPHFCKSALARYTQWDFIDLVGKYGIAWHEKTLGQLFCDDSAEQIVNLLLAECEK
GGVEIRLRSEIISVERDDEGYTLQLNGETVAAKKLVVASGGLSMPGLGASPFGYKLAEQF
GLKVLPTRAGLVPFTLHKPLLEQLQVLSGVSVPSTITAENGTVFRENLLFTHRGLSGPAV
LQISSYWQPGEFVSINLLPDCHLDDFLDEQRAAHPNQSLKNTLAMQLPKRLVECLQQLGQ
LPDVTLKQLNVREQQALVETLTAWRVQPNGTEGYRTAEVTLGGVDTNELSSRTMEARRVP
GLYFIGEVMDVTGWLGGYNFQWAWASAWACAQALAEQK