Protein Info for BWI76_RS26500 in Klebsiella michiganensis M5al

Annotation: nickel ABC transporter, nickel/metallophore periplasmic binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR02294: nickel ABC transporter, nickel/metallophore periplasmic binding protein" amino acids 22 to 518 (497 residues), 795.7 bits, see alignment E=7.8e-244 PF00496: SBP_bac_5" amino acids 66 to 435 (370 residues), 309 bits, see alignment E=2.5e-96

Best Hits

Swiss-Prot: 86% identical to NIKA_ECOLI: Nickel-binding periplasmic protein (nikA) from Escherichia coli (strain K12)

KEGG orthology group: K02035, peptide/nickel transport system substrate-binding protein (inferred from 86% identity to esc:Entcl_2621)

MetaCyc: 86% identical to nickel ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
7.2.2.i [EC: 7.2.2.i]; 7.2.2.- [EC: 7.2.2.i]

Predicted SEED Role

"Nickel ABC transporter, periplasmic nickel-binding protein NikA (TC 3.A.1.5.3)" in subsystem Transport of Nickel and Cobalt (TC 3.A.1.5.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.2.2.i

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAY4 at UniProt or InterPro

Protein Sequence (522 amino acids)

>BWI76_RS26500 nickel ABC transporter, nickel/metallophore periplasmic binding protein (Klebsiella michiganensis M5al)
MSIIRTTLLALLACVPLLAPAAPNDITTAWPVNVGPLNPHLYTPNQMYAQSMVYEPLVKY
QADGSVKPWLATSWRHSEDGKVWTFTLRDDVTFSNGEAFDAVAAAENFRVVLDNRQRHAW
LELVNQIVDVKALDKSHLQITLKSAYYPFLQELSLPRPFRFIAPSQFKDNETLRGIKAPI
GTGPWVLKESKLNQYDVLVRNDRYWGEKPHIQKITVKVIPDPTTRAVAFETGDIDLLYGN
EGLLPLDTFARFSHNPAYRTQLSQPIETVMLALNSAKAPTNELSVREALNHAVNKKSLID
NALYGTQQVADTLFAPTVPYADIGLKPRRYDPQQAKTLLEKAGWVLPAGKDIREKNGQPL
RVELSFIGTDALSKSMAEIIQADMRQVGVDVALAGEEESSIYARQRDGRFGMIFNRTWGA
PYDPHAFMSSMRVPSHADFQAQQGLADKPVIDKEIGEVLETRDETQRQALYRDILTRLHN
DAVYLPISYVSMMVVARPELGAIPYAPIASEIPFEQIKPVKP