Protein Info for BWI76_RS26475 in Klebsiella michiganensis M5al

Annotation: bifunctional UDP-glucuronic acid oxidase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 661 PF00551: Formyl_trans_N" amino acids 25 to 177 (153 residues), 85.6 bits, see alignment E=9.5e-28 PF02911: Formyl_trans_C" amino acids 202 to 294 (93 residues), 67.7 bits, see alignment E=2.2e-22 PF01370: Epimerase" amino acids 318 to 566 (249 residues), 150.9 bits, see alignment E=1e-47 PF16363: GDP_Man_Dehyd" amino acids 319 to 644 (326 residues), 86.5 bits, see alignment E=6.2e-28 PF01073: 3Beta_HSD" amino acids 319 to 496 (178 residues), 22.2 bits, see alignment E=1.7e-08

Best Hits

Swiss-Prot: 94% identical to ARNA_KLEP7: Bifunctional polymyxin resistance protein ArnA (arnA) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_05690)

MetaCyc: 77% identical to fused UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronate dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN0-1862 [EC: 2.1.2.13]; RXN0-1861 [EC: 2.1.2.13, 1.1.1.305]

Predicted SEED Role

"UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305) / UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13)" (EC 1.1.1.305, EC 2.1.2.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.305 or 2.1.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAW9 at UniProt or InterPro

Protein Sequence (661 amino acids)

>BWI76_RS26475 bifunctional UDP-glucuronic acid oxidase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase (Klebsiella michiganensis M5al)
MKAVVFAYHDMGCAGIQSLLDAGYDIAAIFTHPDNPGENHFFGSVARIAAEQGIPVWAPE
DVNHPLWIERIREIKPDVLFSFYYRNLLCDDILNIAPQGAFNLHGSLLPKYRGRAPLNWV
LVNGESETGVTLHRMVNRADAGNIVAQKSVAIGADDAALALHRKLCAAASDLLAQALPAI
RDGKTEERVQDESQATYVGRRTPEDGRLDWERSAQTLHNLVRAVSDPWPGAFGYAGANKF
IVWKSRVRHDLAAAKAGTVLSVAPLVVACQEGALEIVTGQTERGVYMQGTQLAQALGLVA
GAVLSSKPVVAIKRRTRVLILGVNGFIGNHLTERLLQDDNYEIYGLDIGSDAISRFLDNP
RFHFVEGDISIHSEWIEYHIKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLKIIRDC
VKYDKRIIFPSTSEVYGMCTDNNFDEDTSNLVVGPINKQRWIYSVSKQLLDRVIWAYGDK
NGLKFTLFRPFNWMGPRLDNLNAARIGSSRAITQLILNLVEGSPIKLIEGGKQKRCFTDI
SDGIEALFRIIENKDGRCDGQIINIGNPDNEASIKELAEMLLACFERHPLRDRFPPFAGF
REVESSDYYGKGYQDVEHRKPSIRNAKRCLNWVPTVEMEETVEHTLDFFLRTVELTDSGK
S