Protein Info for BWI76_RS26375 in Klebsiella michiganensis M5al

Annotation: aspartate aminotransferase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00700: 4-aminobutyrate transaminase
  • amino acids 8 to 419 (412 residues), 675.2 bits, E=1.5e-207
• PF00202: Aminotran_3
  • amino acids 20 to 419 (400 residues), 455.1 bits, E=1.9e-140
• PF00155: Aminotran_1_2
  • amino acids 215 to 380 (166 residues), 22.5 bits, E=5.9e-09

Best Hits

Swiss-Prot: 76% identical to PUUE_ECOLI: 4-aminobutyrate aminotransferase PuuE (puuE) from Escherichia coli (strain K12)

KEGG orthology group: K00823, 4-aminobutyrate aminotransferase [EC: 2.6.1.19] (inferred from 92% identity to cro:ROD_43631)

MetaCyc: 76% identical to 4-aminobutyrate aminotransferase PuuE (Escherichia coli K-12 substr. MG1655)
5-aminovalerate transaminase. [EC: 2.6.1.48]; 4-aminobutyrate transaminase. [EC: 2.6.1.48, 2.6.1.19]

Predicted SEED Role

"Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" (EC 2.6.1.19)

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (13/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (11/11 steps found)
• superpathway of 4-aminobutanoate degradation (3/3 steps found)
• L-lysine degradation X (5/6 steps found)
• 4-aminobutanoate degradation I (2/2 steps found)
• 4-aminobutanoate degradation II (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• GABA shunt I (3/4 steps found)
• L-lysine degradation I (5/7 steps found)
• GABA shunt II (2/4 steps found)
• L-lysine degradation IV (2/5 steps found)
• 4-aminobutanoate degradation V (3/7 steps found)
• L-lysine degradation III (2/6 steps found)
• nicotine degradation I (pyridine pathway) (6/17 steps found)
• superpathway of L-lysine degradation (12/43 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Butanoate metabolism
• Glutamate metabolism
• Lysine degradation
• Propanoate metabolism
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.19 or 2.6.1.48

Sequence Analysis Tools

See A0A285BBJ8 at UniProt or InterPro

Protein Sequence (421 amino acids)

>BWI76_RS26375 aspartate aminotransferase family protein (Klebsiella michiganensis M5al)
MKSSELNQRRQQATPRGVGVMCSYFVEKAENATLWDIEGNEVIDFAAGIAVLNTGHRHPK
IIAAVEQQLQAFTHTAYQIVPYESYVTLAERINALAPIDGPAKTAFFTTGAEAVENAVKI
ARAYTGRPGLITFGGGFHGRTFMTMALTGKVAPYKIGFGPFPGSVYHAVYPNAAHGITTA
DAMKSLDRIFKADIAADQVAAIVLEPIQGEGGFNVAPPEFMQALRALCDTHGILLIADEV
QTGFARTGKLFAMQHYDVKPDLMTMAKSLAGGFPLSGVVGRAEVMDAPAPGGLGGTYAGN
PLAVAAAHAVLDVIEEEQLCQRAERLGSHLKEVLNQARQSCPAIADVRGQGSMVAVEFND
PQTGEPSAEITRQIQQKAQENGLLLLSCGVYGNVIRFLYPLTIPDAQFTKALDILARVLK
P