Protein Info for BWI76_RS26350 in Klebsiella michiganensis M5al

Annotation: branched chain amino acid ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 369 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF13458: Peripla_BP_6" amino acids 26 to 363 (338 residues), 202.8 bits, see alignment E=2e-63 PF13433: Peripla_BP_5" amino acids 26 to 363 (338 residues), 68 bits, see alignment E=1.2e-22 PF01094: ANF_receptor" amino acids 47 to 360 (314 residues), 104 bits, see alignment E=1.3e-33

Best Hits

Swiss-Prot: 91% identical to LIVK_SALTI: Leucine-specific-binding protein (livK) from Salmonella typhi

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 94% identity to kpu:KP1_5155)

MetaCyc: 90% identical to L-leucine/L-phenylalanine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-35-RXN [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]

Predicted SEED Role

"High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1)" in subsystem ABC transporter branched-chain amino acid (TC 3.A.1.4.1) (TC 3.A.1.4.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAV4 at UniProt or InterPro

Protein Sequence (369 amino acids)

>BWI76_RS26350 branched chain amino acid ABC transporter substrate-binding protein (Klebsiella michiganensis M5al)
MKRNAKTVIAGLVALAMSQAAFAKDIKVAVVGAMSGPVAQWGDMEFNGARQAIKDINAQG
GIKGDKLVAVEYDDACDPKQAVAVANKIVNDGIQYVIGHLCSSSTQPASDIYEDEGILMI
SPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYIMETVKPQRIAIIHDKQQYGEGLARS
VQESLKKGGANIVFFDGITAGEKDFSALLARLKKENIDFVYYGGYYPEMGQMLRQARSVG
LKTVFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYDQDPANSAIVDALKAEKKDPSGPYV
WITYAAVQSLAQAMDRAASQEPLDLIKDLKAHGAKTVIGPLNWDEKGDLKGFEFGVFQWH
ADGSSTLAK