Protein Info for BWI76_RS26195 in Klebsiella michiganensis M5al

Annotation: glycogen phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 815 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 14 to 810 (797 residues), 1227 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 102 to 810 (709 residues), 1089.6 bits, see alignment E=0

Best Hits

Swiss-Prot: 90% identical to PHSG_ECOLI: Glycogen phosphorylase (glgP) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_05375)

MetaCyc: 90% identical to glycogen phosphorylase (Escherichia coli K-12 substr. MG1655)
Phosphorylase. [EC: 2.4.1.1]

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.1

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAR6 at UniProt or InterPro

Protein Sequence (815 amino acids)

>BWI76_RS26195 glycogen phosphorylase (Klebsiella michiganensis M5al)
MNVPFSYSSPTLSVEALKHSIAYKLMFIIGKDPAIANKHEWLNATLFAVRDRMVERWLRS
NRAQLSQEVRQVYYLSMEFLIGRTLSNALLSLGIYEDVNSALEEMGLNLEELIDEENDPG
LGNGGLGRLAACFLDSLAALGLPGRGYGIRYDYGMFKQNIVDGRQKESPDYWLEYGNPWE
FERHNTRYKVRFGGRIQQEGKYSRWVETEEIIAEAYDQIIPGFDTDATNTLRLWSAQASS
EINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTYSGRELRLRQEYFLVSATVQDILSR
HYQLHKTYANLADKIAIHLNDTHPVLSIPELMRLLIDEHKFSWDEAFEVTCQVFSYTNHT
LMSEALETWPVDMLGKILPRHLQIIFEINDYFLKTLQEQYPNDTAMLGRTSIIDESNGRR
VRMAWLAVVVSHKVNGVSELHSRLMVESLFAEFAKIFPMRFTNVTNGVTPRRWLALANPP
LSKVLDENIGHTWRTDLSQLKELEQHIDYPKVNQAVRQAKLENKQRLANYIGQQLNVVVN
PKALFDVQIKRIHEYKRQLMNVLHVITRYNRIKANPDAEWVPRVNIFAGKAASAYYMAKH
IIHLINDVAAVVNNDPQIGDKLKVVFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSN
MKFALNGALTIGTLDGANVEMQEHVGADNFFIFGNTAEEVEALRADGYKPRDYYEQDEEL
HQALTQIGTGLFSPSEPGRYRDLLDSLINFGDHYQVLADFRSYVDCQDKVDELYLHPEEW
ANKAMLNIANMGYFSSDRTIKEYADHIWHIDPVRL