Protein Info for BWI76_RS26165 in Klebsiella michiganensis M5al

Updated annotation (from data): Glycogen phosphorylase (EC 2.4.1.1)
Rationale: Specifically important for utilizing a-Cyclodextrin; D-Maltose monohydrate. Automated validation from mutant phenotype: the predicted function (2.4.1.1) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: maltodextrin phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 796 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 8 to 793 (786 residues), 1095.8 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 93 to 793 (701 residues), 1038.8 bits, see alignment E=0

Best Hits

Swiss-Prot: 88% identical to PHSM_ECOLI: Maltodextrin phosphorylase (malP) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 94% identity to eae:EAE_05345)

MetaCyc: 88% identical to maltodextrin phosphorylase (Escherichia coli K-12 substr. MG1655)
Phosphorylase. [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]

Predicted SEED Role

"Maltodextrin phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.1

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAW6 at UniProt or InterPro

Protein Sequence (796 amino acids)

>BWI76_RS26165 Glycogen phosphorylase (EC 2.4.1.1) (Klebsiella michiganensis M5al)
MSQTSFNKAQFQAALTRQWQHFGLQSASEMTQRQWWRAVSGALSELLSAQPVAKATEGER
HVNYISMEFLIGRLTGNNLLNLGWYQQVSDELQAHDVNLTDLLEEEIDPGLGNGGLGRLA
ACFLDSMATVGQSATGYGLNYQYGLFRQSFADGQQMEAPDDWGRNSYPWFRHNEALDVQV
GLGGKVTKGGEWVPAFVITGEAWDLPVLGYRNNVAQPLRLWQAKHAHPFNLTKFNDGDFL
RAEQQGIDAEKLTKVLYPNDNHQAGKKLRLMQQYFQCACSVADILRRHHLAGRKLAELAD
YEVIQLNDTHPTIAIPELLRVLIDEHQLSWDEAWAITSKTFAYTNHTLMPEALECWDEKL
VKALLPRHMQIIKEINDRFKTLVDKTWPGDKQVWAKLAVVHDKQVRMANMCVVSGFAVNG
VAALHSDLVVKDLFPEYHQLWPNKFHNVTNGITPRRWIKQCNPALAALLDKTLKKEWAND
LDQLINLEKYADDAKFRQTYRDIKLANKVRLAEFVKRRTGIEINPQAIFDIQIKRLHEYK
RQHLNLLNILAQYKEIRENPQADRVPRVFLFGAKAAPGYYLAKNIIWAINKVAAAINNDP
LVGDKLKVVFLPDYCVSAAEMLIPAADVSEQISTAGKEASGTGNMKLALNGALTVGTLDG
ANVEIAEQVGEENIFIFGHTVEEVKALKAKGYDPLKWRKKDKLLDAVLKELESGKYSDGD
KHAFDQMLHSLLKGGDPYLVLADFEAYVAAQKQVDELYRDQEAWTRATILNTARCGMFSS
DRSIRDYQQRIWQAKR