Protein Info for BWI76_RS26155 in Klebsiella michiganensis M5al

Annotation: phosphoenolpyruvate carboxykinase (ATP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 540 TIGR00224: phosphoenolpyruvate carboxykinase (ATP)" amino acids 4 to 539 (536 residues), 962.1 bits, see alignment E=4.4e-294 PF01293: PEPCK_ATP" amino acids 22 to 491 (470 residues), 701.1 bits, see alignment E=3.3e-215

Best Hits

Swiss-Prot: 93% identical to PCKA_ECOLC: Phosphoenolpyruvate carboxykinase (ATP) (pckA) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: K01610, phosphoenolpyruvate carboxykinase (ATP) [EC: 4.1.1.49] (inferred from 94% identity to kpe:KPK_0343)

MetaCyc: 93% identical to phosphoenolpyruvate carboxykinase (ATP) (Escherichia coli K-12 substr. MG1655)
Phosphoenolpyruvate carboxykinase (ATP). [EC: 4.1.1.49]

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP or Serine-glyoxylate cycle (EC 4.1.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.49

Use Curated BLAST to search for 4.1.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BB56 at UniProt or InterPro

Protein Sequence (540 amino acids)

>BWI76_RS26155 phosphoenolpyruvate carboxykinase (ATP) (Klebsiella michiganensis M5al)
MRVNNGLTPQDLKAYGINDVQDIVHNPSYDTLYQEELAPNLQGYERGVLTNLGAIAVDTG
IFTGRSPKDKYIVRDDTTRDTVWWADKGKGKNDNKPLSEETWQHLKGLVTRQLSGKRLFI
VDAFCGANADTRLSVRFITEVAWQAHFVKNMFIRPSDEELANFKPDFIVMNGAKCTNPQW
KEQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSIMNYLLPLKGIASMHCSANVGEK
EDVAIFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEAAEPDI
YHAIRRDALLENVVVLADGTIDFNDGSKTENTRVSYPINHIENIVKPISKAGHATKVIFL
TADAFGVLPPVSRLTADQTQYHFLSGFTAKLAGTERGVTEPTPTFSACFGAAFLSLHPTQ
YAEVLVKRMQASGAQAYLVNTGWNGTGKRISIKDTRAIIDAILDGSLDNAETFTLPMFEL
QIPTELPGVDTHILDPRNTYGSPEQWQEKAEQLAKLFIENFEKYTDTPAGAALVAAGPKR