Protein Info for BWI76_RS26040 in Klebsiella michiganensis M5al

Annotation: nitrite reductase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 847 PF07992: Pyr_redox_2" amino acids 5 to 294 (290 residues), 189.7 bits, see alignment E=2.8e-59 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 801 (796 residues), 1356.5 bits, see alignment E=0 PF13738: Pyr_redox_3" amino acids 83 to 280 (198 residues), 34.8 bits, see alignment E=3.9e-12 PF00070: Pyr_redox" amino acids 148 to 227 (80 residues), 65.5 bits, see alignment E=1.7e-21 PF18267: Rubredoxin_C" amino acids 321 to 387 (67 residues), 37.5 bits, see alignment E=6.8e-13 PF04324: Fer2_BFD" amino acids 424 to 471 (48 residues), 53.8 bits, see alignment 7.2e-18 PF03460: NIR_SIR_ferr" amino acids 560 to 622 (63 residues), 42 bits, see alignment 2.3e-14 PF01077: NIR_SIR" amino acids 633 to 757 (125 residues), 68.7 bits, see alignment E=1.4e-22

Best Hits

Swiss-Prot: 96% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 97% identity to cko:CKO_04789)

MetaCyc: 96% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BB34 at UniProt or InterPro

Protein Sequence (847 amino acids)

>BWI76_RS26040 nitrite reductase large subunit (Klebsiella michiganensis M5al)
MSKVRLAIIGNGMVGHRFIEDLLDKSDANLFDITVFCEEPRIAYDRVHLSSYFSHHTAEE
LSLVREGFYEKHGVKVLVGERAITINRQEKVIHSSAGRTVFYDKLIMATGSYPWIPPIKG
SETQDCFVYRTIEDLNAIEACARRSKRGAVVGGGLLGLEAAGALKNLGVETHVIEFAPML
MAEQLDQMGGEQLRRKIESMGVRVHTSKNTKEIVQEGTQARKTMRFADGSELEVDFIVFS
TGIRPRDKLATQCGLEVAPRGGIVINDACQTSDPDIYAIGECASWNNRVFGLVAPGYKMA
QVTVDHILQTENAFEGADLSAKLKLLGVDVGGIGDAHGRTPGARSYVYLDESKEVYKRLI
VSPDNKTLLGAVLVGDTSDYGNLLQLVLNAIELPENPDSLILPAHAGSGKPSIGVDKLPD
SAQICSCFDVTKGALIAAINKGCHTVAALKAETKAGTGCGGCIPLVTQVLNAELAKQGIE
VNNNLCEHFAYSRQELFHLIRVEGIKTFEELLAKHGKGYGCEVCKPTVGSLLASCWNEYI
LKPQHTPLQDTNDNFLANIQKDGTYSVIPRSAGGEITPEGLVAVGRIAREFNLYTKITGS
QRIGLFGAQKDDLPEIWRQLIEAGFETGHAYAKALRMAKTCVGSTWCRYGVGDSVGFGVE
LENRYKGIRTPHKMKFGVSGCTRECAEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLL
AADLDRETLMQYLDRFMMFYIRTADKLTRTAPWLDNLEGGIDYLKSVIIDDKLGLNAHLE
EEMARLREAVVCEWTETVNTPAAQVRFKHFINSDKRDPNVQVVPEREQHRPAAPYERIPV
TLVEENA