Protein Info for BWI76_RS25675 in Klebsiella michiganensis M5al

Annotation: methionyl-tRNA formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 315 TIGR00460: methionyl-tRNA formyltransferase" amino acids 5 to 312 (308 residues), 406.9 bits, see alignment E=2.8e-126 PF00551: Formyl_trans_N" amino acids 6 to 184 (179 residues), 193.2 bits, see alignment E=3.6e-61 PF02911: Formyl_trans_C" amino acids 207 to 304 (98 residues), 109.8 bits, see alignment E=6.3e-36

Best Hits

Swiss-Prot: 91% identical to FMT_ECO57: Methionyl-tRNA formyltransferase (fmt) from Escherichia coli O157:H7

KEGG orthology group: K00604, methionyl-tRNA formyltransferase [EC: 2.1.2.9] (inferred from 91% identity to eco:b3288)

MetaCyc: 91% identical to 10-formyltetrahydrofolate:L-methionyl-tRNAfMet N-formyltransferase (Escherichia coli K-12 substr. MG1655)
Methionyl-tRNA formyltransferase. [EC: 2.1.2.9]; 2.1.2.9 [EC: 2.1.2.9]

Predicted SEED Role

"Methionyl-tRNA formyltransferase (EC 2.1.2.9)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or Folate Biosynthesis (EC 2.1.2.9)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAG4 at UniProt or InterPro

Protein Sequence (315 amino acids)

>BWI76_RS25675 methionyl-tRNA formyltransferase (Klebsiella michiganensis M5al)
MSDSLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRGKKLMPSPVKVLAEEQG
IPVFQPASLRPQENQQLVSDLRADVMVVVAYGLLLPKAVLDIPRLGCINVHGSLLPRWRG
AAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLACPITAEDTSGTLYDKLANLGPQGL
IETLKQLAAGTAKPVVQDEALVTHAQKLSKEEARIDWSLSAAQLERCIRAFNPWPMSWIE
IDGQPVKVWQASAIDTATKAEPGTILEATKRGIQVATGDGVLNLLSLQPAGKKAMSAQDL
LNSRREWFVPGSRLV