Protein Info for BWI76_RS25665 in Klebsiella michiganensis M5al

Annotation: DNA-processing protein DprA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 TIGR00732: DNA protecting protein DprA" amino acids 64 to 282 (219 residues), 259 bits, see alignment E=1.3e-81 PF02481: DNA_processg_A" amino acids 67 to 274 (208 residues), 244.4 bits, see alignment E=7.1e-77 PF17782: DprA_WH" amino acids 312 to 357 (46 residues), 38.7 bits, see alignment 8.6e-14

Best Hits

Swiss-Prot: 62% identical to SMF_ECOLI: Protein Smf (smf) from Escherichia coli (strain K12)

KEGG orthology group: K04096, DNA processing protein (inferred from 74% identity to kva:Kvar_0418)

Predicted SEED Role

"Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAR2 at UniProt or InterPro

Protein Sequence (374 amino acids)

>BWI76_RS25665 DNA-processing protein DprA (Klebsiella michiganensis M5al)
MTPREIWLRLMRVSSLYGEGAISAARQLCSSPTLEREELRARGLNLAQSKHFLGVNQCEI
DATLQWLERPNCYLLTAEDPLYPPQLRAIVDFPCALLVCGDPALLSSRQLSVIGSRSHSW
YGARWGKLFSEALARYGITITSGLALGIDGIAHRGALEGKGKTVAVLGNGLGEIYPRRHQ
ALAEQIINGGGAVISEFPLFTPPLPGNFPRRNRIISGLSAGVLVIEAALKSGSLVTVRYA
LEQGRDVFALPGPIGSPGSEGPHWLIKQGAIPVTAPEDIVEYWRDELPYILPEPDEVNNC
GNRPSVALPFPELLANVGDEVTPVDVVAERAGQSVPVTVAQLLELELAGWIAAVPGGYVR
LRRASHVRRTDVSV