Protein Info for BWI76_RS25585 in Klebsiella michiganensis M5al
Annotation: multidrug efflux RND transporter permease subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 86% identical to ACRF_ECOLI: Multidrug export protein AcrF (acrF) from Escherichia coli (strain K12)
KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 69% identity to abo:ABO_0964)MetaCyc: 81% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592
Predicted SEED Role
"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285BAU1 at UniProt or InterPro
Protein Sequence (1036 amino acids)
>BWI76_RS25585 multidrug efflux RND transporter permease subunit (Klebsiella michiganensis M5al) MSKFFIHRPVFAWVLAIIMMIAGGLAIMQLPIAQYPTIAPPAVAISATYPGADAQTVQDT VTQVIEQNMNGIDNLMYMSSTSDSAGGVTITLTFTSGTDPDIAQVQVQNKLQLATPLLPQ EVQQQGISVEKSSSSFLMVAGFISDNASTTQDDISDYVASNVKDTISRLNGVGDVQLFGA QYAMRVWLDGNLLNKYNLTPVDVINQLQIQNDQIAAGQLGGTPALKGQQLNASIIAQTRL KDPAEFGKVTLRVNADGSVVHLKDVARIELGGENYNVVAKINGKPASGLGIKLATGANAL DTAAAIKAKLAELQPYFPQGMKVVYPYDTTPFVKISIHEVVKTLFEAIILVFLVMYLFLQ NIRATLIPTIAVPVVLLGTFAVLATFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM VEEKLPPKEATEKSMAQIQGALVGIAMVLSAVFVPMAFFGGSTGAIYRQFSITIVSAMAL SVLVALILTPALCATLLKPASADHHEKKGFFGWFNAKFDRSVSHYTNSVSGILRCTGRYL IIYLLIVVGMAVLFVRLPTSFLPDEDQGVFMTMIQLPSGATQERTQKVLDTVTDYYLHKE KANVESVFTVNGFSFSGQGQNSGMAFVSLKPWEERSGSENGVEAIIKRATAAFSQIKDAM VFPFNLPAIIELGTATGFDFELIDQGNLGHSALTQARNQLLGMVREHPDQLVRVRPNGLE DTPQFKLDVDQEKAQALGISLSDINETISAALGGYYVNDFIDHGRVKKVYVQADAKFRML PSDINNMYVRSANGEMVPFSAFVTSRWVYGSPRLERYNGLPSMEMLGEAAPGKSTGDAMI LMEQLASKLPTGIGYDWTGMSYQERLSGNQAPALYAISLIVVFLCLAALYESWSIPFSVM LVVPLGVVGALLAASLRGLNNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGIIE ATLEASRMRLRPILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMLMATLLAIFFV PVFFVVVRRRFTKHAE