Protein Info for BWI76_RS25580 in Klebsiella michiganensis M5al

Annotation: MexX family efflux pump subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00529: CusB_dom_1" amino acids 37 to 355 (319 residues), 62 bits, see alignment E=8.3e-21 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 38 to 369 (332 residues), 282.9 bits, see alignment E=1.5e-88 PF16576: HlyD_D23" amino acids 61 to 287 (227 residues), 53.4 bits, see alignment E=4.4e-18 PF13533: Biotin_lipoyl_2" amino acids 63 to 107 (45 residues), 25.1 bits, see alignment 2.4e-09 PF13437: HlyD_3" amino acids 173 to 284 (112 residues), 31 bits, see alignment E=6.9e-11

Best Hits

Swiss-Prot: 73% identical to ACRE_ECOLI: Multidrug export protein AcrE (acrE) from Escherichia coli (strain K12)

KEGG orthology group: K03585, membrane fusion protein (inferred from 88% identity to kpe:KPK_0445)

MetaCyc: 73% identical to multidrug efflux pump membrane fusion lipoprotein AcrE (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-367

Predicted SEED Role

"RND efflux system, membrane fusion protein CmeA" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAE4 at UniProt or InterPro

Protein Sequence (379 amino acids)

>BWI76_RS25580 MexX family efflux pump subunit (Klebsiella michiganensis M5al)
MTPHVRVTLLSSLIISAILLSGCDNSGDGQPHAQIPQVSVYVVHSAPLSVTTELPGRTSA
YRVAEVRPQVNGIILQRNFVEGSDVSAGQSLYQIDPATYQAAYNSTKGDEAKAEAAAAIA
HLTVKRYAPLLGTKYISQQEYDQAVATARQADADVIAAKAAVESARINLAYTKVTSPISG
RIGKSSVTEGALVTNGQADAMATVQQLDPIYVDVTESSNDFMRLKQESLQHGNDTKNVQL
IMENGQPYTLQGALQFSDVTVDESTGSITLRAIFPNPQHALLPGMFVRARIDEGVSPNAI
LVPQQGVTRTPRGEASVMLVNDKNQVETRAVTAAQAIGDKWLVTSGLKAGEKVIVSGLQK
VRPGVTVKAEEDTATSVAQ