Protein Info for BWI76_RS25575 in Klebsiella michiganensis M5al

Annotation: acrEF/envCD operon transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 216 PF00440: TetR_N" amino acids 16 to 62 (47 residues), 62.5 bits, see alignment 2.6e-21 PF08361: TetR_C_2" amino acids 84 to 201 (118 residues), 127.3 bits, see alignment E=3.6e-41

Best Hits

Swiss-Prot: 53% identical to ENVR_ECO57: Probable acrEF/envCD operon repressor (envR) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 73% identity to kva:Kvar_0428)

Predicted SEED Role

"Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (216 amino acids)

>BWI76_RS25575 acrEF/envCD operon transcriptional regulator (Klebsiella michiganensis M5al)
MPRRTKEDALKTRQLLIESAIQQFALRGVTSTTLADIADAAGVTRGAVYWHFASKAELFN
EIWQQQLPLRDLLQHEFPQKESKDPLSTLREKFIIGLQYIANNPRQRALMQILYQKCEFS
SDMMPEAEIRKRIGFSYPIIGGVLQLCIRNGVLPAETNVEISLIVLHSAFTGIIKNWLVE
PGQFDLYRQAPALVDNIMSILCGTTRQLATVSHYNQ