Protein Info for BWI76_RS25450 in Klebsiella michiganensis M5al

Annotation: p-hydroxybenzoic acid efflux subunit AaeA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details PF00529: CusB_dom_1" amino acids 22 to 285 (264 residues), 42.1 bits, see alignment E=1.4e-14 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 46 to 241 (196 residues), 110.2 bits, see alignment E=5e-36 PF16576: HlyD_D23" amino acids 46 to 235 (190 residues), 51.7 bits, see alignment E=1.5e-17 PF13533: Biotin_lipoyl_2" amino acids 48 to 94 (47 residues), 61.3 bits, see alignment 1.2e-20 PF13437: HlyD_3" amino acids 156 to 250 (95 residues), 39.7 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 93% identical to AAEA_KLEP3: p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: None (inferred from 92% identity to kpu:KP1_4966)

MetaCyc: 84% identical to aromatic carboxylic acid efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-233

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BB25 at UniProt or InterPro

Protein Sequence (310 amino acids)

>BWI76_RS25450 p-hydroxybenzoic acid efflux subunit AaeA (Klebsiella michiganensis M5al)
MKTLTRNILRTAITVILVILAFVAIFRAWVYYTASPWTRDARFSADIVAIAPDVSGLISQ
VNIKDNQLVKKDQVLFVIDQPRYQKALAEAEADVSYYQTLAQEKRVEAGRRNRLGVQAMS
REEIDQANNVLQTVEHQLAKATASRDLAKLDLERTVIRAPADGWVTNLNVYSGEFITRGS
TAVALVKQNTFYVMAYLEETKLEGVRPGFRAEITPLGSVQTLKGTVDSIAAGVTNASSSS
DTKGLASIDSNLEWVRLAQRVPVRIHLDQQQGNLWPAGTTATVVITGKDDRDSSRTSFFQ
KLAMRLREFG