Protein Info for BWI76_RS25370 in Klebsiella michiganensis M5al

Annotation: outer membrane-stress sensor serine endopeptidase DegS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 352 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR02038: periplasmic serine peptidase DegS" amino acids 4 to 349 (346 residues), 562.2 bits, see alignment E=2.3e-173 PF00089: Trypsin" amino acids 66 to 238 (173 residues), 77.1 bits, see alignment E=4.5e-25 PF13365: Trypsin_2" amino acids 77 to 212 (136 residues), 108.3 bits, see alignment E=1.5e-34 PF00595: PDZ" amino acids 252 to 333 (82 residues), 44.8 bits, see alignment E=3.3e-15 PF13180: PDZ_2" amino acids 254 to 344 (91 residues), 55.4 bits, see alignment E=1.6e-18 PF17820: PDZ_6" amino acids 280 to 322 (43 residues), 42.5 bits, see alignment 1.2e-14

Best Hits

Swiss-Prot: 87% identical to DEGS_ECO57: Serine endoprotease DegS (degS) from Escherichia coli O157:H7

KEGG orthology group: K04691, serine protease DegS [EC: 3.4.21.-] (inferred from 96% identity to kpn:KPN_03634)

MetaCyc: 87% identical to serine endoprotease (Escherichia coli K-12 substr. MG1655)
3.4.21.-

Predicted SEED Role

"Outer membrane stress sensor protease DegS" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAA3 at UniProt or InterPro

Protein Sequence (352 amino acids)

>BWI76_RS25370 outer membrane-stress sensor serine endopeptidase DegS (Klebsiella michiganensis M5al)
MPGKLLRSILIGLIVGGLLLAVMPSLRQWQLSPTTQYDSADESPASYNSAVRRAAPAVVN
VYNRALNSTSHNQLTLGSGVIMDQRGYILTNKHVINDADQIIVALQDGRVFEALLVGSDT
LTDLAVLKINASGGLPVIPINPKRVPHIGDVVLAIGNPYNLGQTITQGIISATGRIGLNP
TGRQNFLQTDASINHGNSGGALVNSLGELMGINTLSFDKSNDGETPEGIGFAIPFQLATK
IMDKLIRDGRVIRGYIGISGREIAPLHAQGGGIDQLQGIVVNDVAPEGPAAQAGIRANDV
IISVNDKPAVSALETMDQVAEIRPGSEIPVVIMRDDKKLTLHIAVQEYPATN