Protein Info for BWI76_RS25320 in Klebsiella michiganensis M5al

Annotation: glutamate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01318: glutamate synthase, small subunit
  • amino acids 6 to 472 (467 residues), 878.1 bits, E=5.7e-269
• PF14691: Fer4_20
  • amino acids 25 to 134 (110 residues), 133.3 bits, E=2.1e-42
• PF07992: Pyr_redox_2
  • amino acids 148 to 459 (312 residues), 85.6 bits, E=2.5e-27
• PF01494: FAD_binding_3
  • amino acids 148 to 179 (32 residues), 27.3 bits, see alignment (E = 1.3e-09)
• PF13450: NAD_binding_8
  • amino acids 151 to 185 (35 residues), 33.4 bits, 2.7e-11

Best Hits

Swiss-Prot: 94% identical to GLTD_ECOLI: Glutamate synthase [NADPH] small chain (gltD) from Escherichia coli (strain K12)

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 98% identity to kpe:KPK_0496)

MetaCyc: 94% identical to glutamate synthase subunit GltD (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]; Glutamate synthase (NADPH). [EC: 1.4.1.4, 1.4.1.13]

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.4

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14 or 1.4.1.4

Sequence Analysis Tools

See A0A285BA89 at UniProt or InterPro

Protein Sequence (472 amino acids)

>BWI76_RS25320 glutamate synthase (Klebsiella michiganensis M5al)
MSQNVYQFIDLQRVDPPKKALKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCP
VHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
NIERYINDKAFEMGWRPDLTGVKQTGKTVAIIGAGPAGLACADVLTRNGVKAVVFDRHPE
IGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNIEVGRDVQLDDLLKDYDAVFLGVG
TYQSMRGGLENEDASGVFDALPFLIANTKQIMGFGETADEPYVSMEGKRVVVLGGGDTAM
DCVRTSIRQNATHVICAYRRDEENMPGSKREVKNAREEGVEFQFNVQPLGIEVNANGKVC
GVKMARTEMGQPDAKGRRRAEIVAGSEHVVPADAVVMAFGFRPHSMEWLAKHSVELDSQG
RVIAPEGSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGILNYLEV