Protein Info for BWI76_RS25175 in Klebsiella michiganensis M5al

Annotation: EamA family transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 22 to 24 (3 residues), see Phobius details amino acids 39 to 56 (18 residues), see Phobius details amino acids 71 to 91 (21 residues), see Phobius details amino acids 102 to 120 (19 residues), see Phobius details amino acids 131 to 151 (21 residues), see Phobius details amino acids 157 to 177 (21 residues), see Phobius details amino acids 189 to 205 (17 residues), see Phobius details amino acids 217 to 236 (20 residues), see Phobius details amino acids 248 to 269 (22 residues), see Phobius details amino acids 281 to 300 (20 residues), see Phobius details PF00892: EamA" amino acids 7 to 142 (136 residues), 98.2 bits, see alignment E=2.2e-32 amino acids 159 to 291 (133 residues), 63.4 bits, see alignment E=1.3e-21

Best Hits

Swiss-Prot: 87% identical to YHBE_SHIFL: Uncharacterized inner membrane transporter YhbE (yhbE) from Shigella flexneri

KEGG orthology group: None (inferred from 92% identity to esc:Entcl_0512)

Predicted SEED Role

"Permease of the drug/metabolite transporter (DMT) superfamily" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BA70 at UniProt or InterPro

Protein Sequence (321 amino acids)

>BWI76_RS25175 EamA family transporter (Klebsiella michiganensis M5al)
MKQQAGIGIILALTTAMCWGALPIAMKQVLEVMEPPTVVFYRFLMASFGLGAILAMKRKL
PPLRIFRKPRWLILLVIATGGLFGNFILFSSSLQYLSPTASQVIGQLSPVGMMVASVFVL
KEKMRGTQVVGALMLVSGLVMFFNTSLIEIFTRLTDYTWGVIFGVGAATVWVCYGVAQKV
LLRRLASQQILFLLYTLCTISLLPLAKPSQVLALSDWQLACLVFCGLNTLVGYGALAEAM
ARWQAAQVSALITLTPLFTLLFSDLLSLAWPDVFARPLLNLMGYLGAFVVVAGAMYSAVG
HRLWGRWRKREAVVPLPRSGE