Protein Info for BWI76_RS24880 in Klebsiella michiganensis M5al
Annotation: PTS galactitol transporter subunit IIA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to PTKA_ECOLI: PTS system galactitol-specific EIIA component (gatA) from Escherichia coli (strain K12)
KEGG orthology group: K02773, PTS system, galactitol-specific IIA component [EC: 2.7.1.69] (inferred from 91% identity to kva:Kvar_0549)MetaCyc: 61% identical to galactitol-specific PTS enzyme IIA component (Escherichia coli EC3132)
TRANS-RXN-161 [EC: 2.7.1.200]; TRANS-RXN-169 [EC: 2.7.1.200, 2.7.1.198]
Predicted SEED Role
"PTS system, galactitol-specific IIA component (EC 2.7.1.69)" in subsystem D-Tagatose and Galactitol Utilization (EC 2.7.1.69)
MetaCyc Pathways
- superpathway of hexitol degradation (bacteria) (18/18 steps found)
- galactitol degradation (5/5 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Fructose and mannose metabolism
- Galactose metabolism
- Glycolysis / Gluconeogenesis
- Nucleotide sugars metabolism
- Starch and sucrose metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.69
Use Curated BLAST to search for 2.7.1.198 or 2.7.1.200 or 2.7.1.69
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285BAR0 at UniProt or InterPro
Protein Sequence (155 amino acids)
>BWI76_RS24880 PTS galactitol transporter subunit IIA (Klebsiella michiganensis M5al) MSQLFVRTGIHFSSSQQALEHIGEEMLARGVVHDSYPLALLEREATFPTGIALEQHAVAI PHCEAVHAKSPAIYLIRPDNPVPFQRADDDGEIDVSLIIALIVENPTAQLQLLRRLFSEL QNPTTLEALLAASQEQLPELFRHSILESEPSVQAL