Protein Info for BWI76_RS24510 in Klebsiella michiganensis M5al

Annotation: urease subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 TIGR01792: urease, alpha subunit" amino acids 3 to 567 (565 residues), 972.3 bits, see alignment E=4.3e-297 PF00449: Urease_alpha" amino acids 3 to 119 (117 residues), 185.8 bits, see alignment E=4.6e-59 PF01979: Amidohydro_1" amino acids 125 to 453 (329 residues), 289 bits, see alignment E=8.6e-90

Best Hits

Swiss-Prot: 94% identical to URE1_KLEP3: Urease subunit alpha (ureC) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K01428, urease subunit alpha [EC: 3.5.1.5] (inferred from 94% identity to ecs:ECs1324)

MetaCyc: 72% identical to urease alpha subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease alpha subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B9M5 at UniProt or InterPro

Protein Sequence (567 amino acids)

>BWI76_RS24510 urease subunit alpha (Klebsiella michiganensis M5al)
MSEISRQAYADMFGPTTGDKVRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGMGQGQM
LSAGCVDLVLTNALIVDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIGAATEIIA
AEGKIVTAGGVDTHIHWICPQQAEEALVSGVTTMIGGGTGPAAGTNATTCTPGPWYISRM
LQAADSLPVNIGLLGKGNGSNPDALREQVAAGVIGLKIHEDWGATPAAIDCALTVADEMD
VQVALHSDTLNESGFVEDTLAAIGGRTIHTFHTEGAGGGHAPDIITACAHPNILPSSTNP
TLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRRETIAAEDVLHDLGAFSLTSSD
SQAMGRVGEVVLRTWQVAHRMKVQRGPLAEESGDNDNFRVKRYIAKYTINPALTHGIAHE
VGSIEAGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDINASIPTPQPVHYRPMFGAL
GSARHRCRLTFLSQAAAENGVAERLNLHSATAVVKGCREVQKADMHHNGLLPNITVNSQT
YEVRIDGELITSEPADVLPMAQRYFLF