Protein Info for BWI76_RS24475 in Klebsiella michiganensis M5al

Annotation: multifunctional CCA tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 PF01743: PolyA_pol" amino acids 3 to 122 (120 residues), 68.9 bits, see alignment E=8e-23 PF12627: PolyA_pol_RNAbd" amino acids 149 to 212 (64 residues), 49.6 bits, see alignment E=4e-17 PF01966: HD" amino acids 231 to 328 (98 residues), 50.2 bits, see alignment E=4.4e-17

Best Hits

Swiss-Prot: 91% identical to CCA_KLEP7: Multifunctional CCA protein (cca) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 91% identity to kpe:KPK_0660)

MetaCyc: 85% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B9L4 at UniProt or InterPro

Protein Sequence (413 amino acids)

>BWI76_RS24475 multifunctional CCA tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase (Klebsiella michiganensis M5al)
MKVYLVGGAVRDALLRLPVKDKDWVVVGATPQQLLDAGYQQVGRDFPVFLHPQSREEYAL
ARTERKSGSGYTGFTCYAAADVTLEQDLLRRDLTINALAQDANGQIIDPYGGQTDLRQRL
LRHVSPAFSEDPLRVLRVARFAARYAHLGFRIADETMALMRAMADAGELAHLTPERVWKE
TESALATRNPQVFFQTLHDCRALKVLFPEVDALYGVPAPAKWHPEIDTGLHTLMTVTMAA
MLSPEVDVRFATLCHDLGKGLTPKALWPRHHGHGPAGVKLVEQLCARLRVPNDIRDLAKL
VAEFHDLIHTLPILQPKTLVKLFDNIDAWRKPHRVRQIALTSEADVRGRTGFEACDYPQG
RLLLEAWEVARSVSTKEVLAEGFQGVEIREELTRRRIQAVARWKEKRCPQPRD