Protein Info for BWI76_RS24385 in Klebsiella michiganensis M5al

Annotation: phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00149: Metallophos
  • amino acids 16 to 206 (191 residues), 75.7 bits, E=3.4e-25

Best Hits

Swiss-Prot: 82% identical to CPDA_ECO57: 3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA (cpdA) from Escherichia coli O157:H7

KEGG orthology group: K03651, Icc protein (inferred from 94% identity to kpu:KP1_4727)

MetaCyc: 82% identical to cAMP phosphodiesterase (Escherichia coli K-12 substr. MG1655)
3',5'-cyclic-AMP phosphodiesterase. [EC: 3.1.4.53]

Predicted SEED Role

"3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17)" in subsystem cAMP signaling in bacteria (EC 3.1.4.17)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.17

Use Curated BLAST to search for 3.1.4.17 or 3.1.4.53

Sequence Analysis Tools

See A0A285B9K0 at UniProt or InterPro

Protein Sequence (275 amino acids)

>BWI76_RS24385 phosphodiesterase (Klebsiella michiganensis M5al)
MESLLNLPLAGEARVRILQITDTHLFAEKHETLLGINTWESYQAVLDAIHASQRECDLIV
ATGDLAQDHSAAAYQHFAEGIASFAAPCVWLPGNHDFQPAMYSTLQESGISPAKRVLLGD
HWQILLMDSQVFGVPHGELSDFQLEWLEQKLAEVPERYTLLLLHHHPMPSGCSWLDQHSL
RNAGALDCALAAFPRVKHLLCGHIHQELDLDWNGRRMMATPSTCVQFKPHCANFTLDTVS
PGWRWLDLHPDGTLTTEVCRLEGVKFHPDIASEGY