Protein Info for BWI76_RS24375 in Klebsiella michiganensis M5al

Annotation: DNA topoisomerase IV subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 TIGR01055: DNA topoisomerase IV, B subunit" amino acids 5 to 626 (622 residues), 1207.9 bits, see alignment E=0 PF02518: HATPase_c" amino acids 29 to 171 (143 residues), 53.4 bits, see alignment E=6.6e-18 PF00204: DNA_gyraseB" amino acids 219 to 385 (167 residues), 141.3 bits, see alignment E=4.7e-45 PF01751: Toprim" amino acids 414 to 522 (109 residues), 46.5 bits, see alignment E=6.7e-16 PF00986: DNA_gyraseB_C" amino acids 557 to 621 (65 residues), 82 bits, see alignment E=5.6e-27

Best Hits

Swiss-Prot: 94% identical to PARE_ECOLI: DNA topoisomerase 4 subunit B (parE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 97% identity to eae:EAE_03570)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B9J3 at UniProt or InterPro

Protein Sequence (631 amino acids)

>BWI76_RS24375 DNA topoisomerase IV subunit B (Klebsiella michiganensis M5al)
MTQSSYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVEVIL
HADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVV
NALSKRVEVNVRRDGQIYSIAFENGDKVEDLHVTGTCGKRNTGTSVHFWPDESFFDSPRF
SVSRLSHLLKAKAVLCPGVEIVFRDLVNDSEQRWCYADGLTDYLSEAVNGLPLLPEKPFV
GTFSGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRN
ILPRGVKLSAEDIWERCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLN
QNVQSAELLAEMAISSAQRRLRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEG
DSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQ
LRYGKICILADADSDGLHIATLLCALFVRHFRTLVKHGHVYVALPPLYRIDLGKEVYYAL
TEEEKAGVLEQLKRKKGKPNVQRFKGLGEMNPLQLRETTLDPNTRRLVQLIINDEDEQQT
TAMMDMLLAKKRSEDRRNWLQEKGDLADIEG