Protein Info for BWI76_RS24360 in Klebsiella michiganensis M5al

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 transmembrane" amino acids 12 to 37 (26 residues), see Phobius details amino acids 43 to 66 (24 residues), see Phobius details amino acids 78 to 98 (21 residues), see Phobius details amino acids 107 to 127 (21 residues), see Phobius details amino acids 148 to 165 (18 residues), see Phobius details amino acids 171 to 190 (20 residues), see Phobius details amino acids 222 to 239 (18 residues), see Phobius details amino acids 259 to 283 (25 residues), see Phobius details amino acids 291 to 315 (25 residues), see Phobius details amino acids 321 to 338 (18 residues), see Phobius details amino acids 350 to 370 (21 residues), see Phobius details amino acids 382 to 400 (19 residues), see Phobius details PF01306: LacY_symp" amino acids 6 to 405 (400 residues), 361.8 bits, see alignment E=6.7e-112 TIGR00882: oligosaccharide:H+ symporter" amino acids 11 to 401 (391 residues), 359.5 bits, see alignment E=1.1e-111 PF12832: MFS_1_like" amino acids 12 to 383 (372 residues), 91.8 bits, see alignment E=7.3e-30 PF07690: MFS_1" amino acids 18 to 258 (241 residues), 47 bits, see alignment E=2.8e-16

Best Hits

KEGG orthology group: K02532, MFS transporter, OHS family, lactose permease (inferred from 86% identity to cko:CKO_04419)

Predicted SEED Role

"Sucrose permease, major facilitator superfamily" in subsystem Sucrose utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B9J6 at UniProt or InterPro

Protein Sequence (422 amino acids)

>BWI76_RS24360 MFS transporter (Klebsiella michiganensis M5al)
MESTYTNNKVEYYKISAFIFLYFFTWSASIGLLAIWLGQKANLSGAVIGTVFAVNGVFSV
ILKPIYGYILDKIGMSKYLLYFVVLMSALMAPFFIYVYQPLLLTKTMLGIIVGAIYLSFA
WYAGVAACESYTDRYSRLNNMEFGQIRMWGSLGWAVASSFSGLLFNLSPAYNFILGSVTS
IVMLAVLLSLKVNENAENAAQVLVKDKISPADVYTLLRNRKFWAFCLYVAGVAWMMFIAE
QQFSRYFVTFFADVHKGNAIFGYLGTVQSGMEFIMYMVIPFFVNYIGAKRGLLIVGLLVG
ARLVISGLCDSHLLISLLKPLYGLEICLLLVSVFKYIAEHFDKRVNATMYLLGYQAMLYV
GNVVVSSPAGFMYDRIGFEKTYIIMGMIALVFTVISAFTLSSCQSKRRSSATLNVSENNA
SK