Protein Info for BWI76_RS24355 in Klebsiella michiganensis M5al

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07470: Glyco_hydro_88
  • amino acids 61 to 255 (195 residues), 34.7 bits, E=5.1e-13

Best Hits

Swiss-Prot: 46% identical to UCDH_STRR6: Unsaturated chondroitin disaccharide hydrolase (ugl) from Streptococcus pneumoniae (strain ATCC BAA-255 / R6)

KEGG orthology group: None (inferred from 85% identity to cko:CKO_04418)

MetaCyc: 45% identical to unsaturated beta-glucuronyl hydrolase (Streptococcus agalactiae)
RXN-12177 [EC: 3.2.1.180]; RXN-16485 [EC: 3.2.1.180, 3.2.1.179]; 3.2.1.179 [EC: 3.2.1.180, 3.2.1.179]

Predicted SEED Role

"Unsaturated glucuronyl hydrolase (EC 3.2.1.-)" (EC 3.2.1.-)

MetaCyc Pathways

• gellan degradation (1/2 steps found)
• hyaluronan degradation (1/2 steps found)
• dermatan sulfate degradation I (bacterial) (1/4 steps found)
• chondroitin sulfate degradation I (bacterial) (2/8 steps found)

KEGG Metabolic Maps

• Ascorbate and aldarate metabolism
• Glycosaminoglycan degradation
• Nucleotide sugars metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 3.2.1.179 or 3.2.1.180

Sequence Analysis Tools

See A0A285BA76 at UniProt or InterPro

Protein Sequence (396 amino acids)

>BWI76_RS24355 hypothetical protein (Klebsiella michiganensis M5al)
MLNNIVEEPLRDFPGEPISMDCFREEMKRAREDVLELIGRHLTEFGEQFPAETCVEGYYP
LTENVEWTTSFWTGQLWLAWEMSGEDKFRQMAEKNVRTFGLRIAGRHDTNTHDLGFLYTL
SCVAAWRLTGNRDARGFSLMAAEALLERFHAKAQIIQAWGDLNDPAQAGRMIIDCTMNLP
LLYWASEQTGDPRFRDAALAHVRQSAAFLVREDASTWHTYYMDVVSGKPRYGNTQQGYAD
DSCWSRGQAWGIYGFLLSYIYTGDRALVGLAKKLANYFLNRLPEDYVCHWDLALINTDAL
RDSSSAAIAVCGLLELVKQLPTTDPDRERYQQWAMAIMASLTKGYLMAKSDKGNGLLKHS
VYHLASNKGVDECCSWGDYFYVEALMRMTQSWKLYW