Protein Info for BWI76_RS24170 in Klebsiella michiganensis M5al

Annotation: nucleoside permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 418 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 38 to 61 (24 residues), see Phobius details amino acids 70 to 89 (20 residues), see Phobius details amino acids 95 to 116 (22 residues), see Phobius details amino acids 135 to 155 (21 residues), see Phobius details amino acids 161 to 182 (22 residues), see Phobius details amino acids 211 to 231 (21 residues), see Phobius details amino acids 250 to 275 (26 residues), see Phobius details amino acids 282 to 300 (19 residues), see Phobius details amino acids 306 to 327 (22 residues), see Phobius details amino acids 347 to 365 (19 residues), see Phobius details amino acids 383 to 402 (20 residues), see Phobius details PF03825: Nuc_H_symport" amino acids 1 to 406 (406 residues), 605 bits, see alignment E=1.1e-185 TIGR00889: nucleoside transporter" amino acids 1 to 418 (418 residues), 716 bits, see alignment E=8.1e-220 PF12832: MFS_1_like" amino acids 6 to 373 (368 residues), 88.8 bits, see alignment E=6e-29 PF07690: MFS_1" amino acids 252 to 404 (153 residues), 42.8 bits, see alignment E=5.2e-15

Best Hits

Swiss-Prot: 90% identical to NUPG_SHIFL: Nucleoside permease NupG (nupG) from Shigella flexneri

KEGG orthology group: K03289, MFS transporter, NHS family, nucleoside permease (inferred from 94% identity to cko:CKO_04339)

MetaCyc: 90% identical to nucleoside:H+ symporter NupG (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-108A; TRANS-RXN-108B; TRANS-RXN-108C; TRANS-RXN-108D; TRANS-RXN-108E; TRANS-RXN-108G; TRANS-RXN-108H; TRANS-RXN-108I; TRANS-RXN-474; TRANS-RXN-475; TRANS-RXN-476

Predicted SEED Role

"Nucleoside permease NupG"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B9Z7 at UniProt or InterPro

Protein Sequence (418 amino acids)

>BWI76_RS24170 nucleoside permease (Klebsiella michiganensis M5al)
MNLKLQLKVLSFLQFCLWGSWLTTLGSYMFVTLKFDGAAIGAVYSSLGIAAVFMPTLLGI
VADKWISAKWVYAICHLVGALTLWLAAQVTTPGEMFLVILLNSLAYMPTLGLINTISYYR
LQSADMDIVTDFPPIRIWGTIGFILAMWAVSFSGFELSHMQLYIGAALSLLLVLFTLTLP
HIPVANQQKNQSWTAMLGLDAFALFKNKRMAIFFIFSMMLGAELQITNMFGNTFLHSFDK
DPLFASSFIVQHASVMMSISQISETLFILTIPFFLSRYGIKNVMLISIVAWMLRFGLFAY
GDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVEKEVRPEIRASAQGMFLMMTNGFGCIL
GGMVSGKVVEHFTVEGITDWQTVWLIFAGYSLVLAFAFVALFKYKHVRVPTGAQTVAH