Protein Info for BWI76_RS24165 in Klebsiella michiganensis M5al

Annotation: lytic murein transglycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF11873: Mltc_N" amino acids 33 to 193 (161 residues), 236.1 bits, see alignment E=1.7e-74 PF01464: SLT" amino acids 197 to 321 (125 residues), 108.6 bits, see alignment E=1.5e-35

Best Hits

Swiss-Prot: 94% identical to MLTC_KLEP3: Membrane-bound lytic murein transglycosylase C (mltC) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K08306, membrane-bound lytic murein transglycosylase C [EC: 3.2.1.-] (inferred from 94% identity to kpe:KPK_0714)

MetaCyc: 87% identical to membrane-bound lytic murein transglycosylase C (Escherichia coli K-12 substr. MG1655)
4.2.2.f [EC: 4.2.2.f]; 4.2.2.f [EC: 4.2.2.f]

Predicted SEED Role

"Membrane-bound lytic murein transglycosylase C precursor (EC 3.2.1.-)" (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 4.2.2.f

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B9I3 at UniProt or InterPro

Protein Sequence (361 amino acids)

>BWI76_RS24165 lytic murein transglycosylase (Klebsiella michiganensis M5al)
MMKKYLALALIAPLLVSCSSSTKKGAEYNEAWVKDTNGFDILMGQFAHNIENIWGYNEVL
LAGPKDYVKYTDQYQTRSHINFDAGTITVETIAGTDPAGRLRQAIIKTLLMGDDPNSIDL
YSDVDDIQISKEPFLYGQVVDNTGEAIRWEWRASRFADYLLQTRLKSRSNGPRMIYSITI
NLVPNHLDKRAHKYIGMVRQASRKYGVDESLILAIMQTESSFNPYAVSHADALGLMQVVQ
HSAGKDVFRSQGKSGLPSRSYLFDPVNNIDTGTAYLAMLNNVYLSGITNPTSRRYAVITA
YNGGAGSVLRVFSSDKVQAANIINNMTPGDVYQTITSRHPSAESRRYLYKVNSAQKGYRR
R