Protein Info for BWI76_RS23935 in Klebsiella michiganensis M5al

Annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01642: MM_CoA_mutase
  • amino acids 28 to 539 (512 residues), 774.5 bits, E=4.8e-237
• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 28 to 547 (520 residues), 921.9 bits, E=8.6e-282
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 583 to 713 (131 residues), 214.3 bits, E=5e-68
• PF02310: B12-binding
  • amino acids 586 to 702 (117 residues), 69.1 bits, E=3.1e-23

Best Hits

Swiss-Prot: 93% identical to SCPA_ECOLI: Methylmalonyl-CoA mutase (scpA) from Escherichia coli (strain K12)

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 95% identity to esc:Entcl_0837)

MetaCyc: 93% identical to methylmalonyl-CoA mutase (Escherichia coli K-12 substr. MG1655)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• conversion of succinate to propanoate (3/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• pyruvate fermentation to propanoate I (5/7 steps found)
• propanoyl CoA degradation I (2/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• 2-oxobutanoate degradation I (2/4 steps found)
• L-glutamate degradation VIII (to propanoate) (7/11 steps found)
• superpathway of L-methionine salvage and degradation (10/16 steps found)
• methylaspartate cycle (12/19 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Sequence Analysis Tools

See A0A285B9K6 at UniProt or InterPro

Protein Sequence (714 amino acids)

>BWI76_RS23935 methylmalonyl-CoA mutase (Klebsiella michiganensis M5al)
MSELQEWQTLANKELSRREKTVDDLVKQTAEGIAVKPLYTEADLDNLEVTGTLPGLPPYV
RGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDNPR
VAGDVGKAGVAIDTVEDMKILFDRIPLDKMSVSMTMNGAVLPVMAFYIVAAEEQGVASEQ
LTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSDYMPRFNTISISGYHMGEAGAN
CVQQVAFTLADGIEYIKAALCAGLKIDDFAPRLSFFFGIGMDLFMNVAMLRAARYLWSEA
VSGFGATNPKSLALRTHCQTSGWSLTEQDPYNNVIRTTIEALGATLGGTQSLHTNAFDEA
LGLPTDFSARIARNTQIIIQEESEICRTVDPLAGSYYVESLTDQIVKQARAIIQQIAEAG
GMAKAIEAGLPKRMIEEASAREQSLIDQGKRVIVGVNKYKLEKEDETAVLEIDNVKVRNE
QIASLEKIRAQRDDVTVKAALNALTHAAQHHENLLAAAINAARVRATLGEISDALEAAFD
RYLVPSQCVTGVIAQSYHQSDTAAGEFDAIVAQTERFLMDNGRRPRILIAKMGQDGHDRG
AKVIASAYSDLGFDVDLSPMFSTPEEIARLAIENDVHVVGASSLAAGHKTLIPELVAALK
KWGREDICVIAGGVIPPQDYAFLQARGVAAIYGPGTPMLESVRDVLTRISQHHD