Protein Info for BWI76_RS23840 in Klebsiella michiganensis M5al

Annotation: hemolysin III family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details amino acids 54 to 74 (21 residues), see Phobius details amino acids 86 to 107 (22 residues), see Phobius details amino acids 113 to 132 (20 residues), see Phobius details amino acids 140 to 159 (20 residues), see Phobius details amino acids 166 to 187 (22 residues), see Phobius details amino acids 196 to 216 (21 residues), see Phobius details PF03006: HlyIII" amino acids 12 to 209 (198 residues), 133 bits, see alignment E=6.5e-43 TIGR01065: channel protein, hemolysin III family" amino acids 15 to 216 (202 residues), 240.4 bits, see alignment E=6.4e-76

Best Hits

Swiss-Prot: 91% identical to YQFA_ECOLI: UPF0073 inner membrane protein YqfA (yqfA) from Escherichia coli (strain K12)

KEGG orthology group: K11068, hemolysin III (inferred from 91% identity to ebr:ECB_02731)

Predicted SEED Role

"COG1272: Predicted membrane protein hemolysin III homolog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B9C4 at UniProt or InterPro

Protein Sequence (219 amino acids)

>BWI76_RS23840 hemolysin III family protein (Klebsiella michiganensis M5al)
MVRKSLITQGYSLAEEIANSISHGIGLVFGIVGLVLLLVQAVDSNAGATAITSYSLYGGS
MILLFLASTLYHAIPHQRAKLWLKKFDHCAIYLLIAGTYTPFLLVGLNSPLSRGLMIVIW
SLALLGILFKLTIAHRFKILSLVTYLTMGWLSLIVVYQLAIKLAVGGVTLLAVGGIIYSL
GVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYVGQV