Protein Info for BWI76_RS23700 in Klebsiella michiganensis M5al

Updated annotation (from data): glycerate CoA-transferase
Rationale: Specifically important in carbon source 2-Deoxy-D-ribonic acid lithium salt. After oxidization and cleavage to glyceryl-CoA, this enzyme would form glycerate. This protein is 42% identical to a propionate CoA-transferase (Q9L3F7) that uses propionyl-CoA and acetate as substrates, so it is likely that acetate is the CoA-acceptor.
Original annotation: acetyl-CoA--acetoacetyl-CoA transferase subunit alpha/beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01144: CoA_trans
  • amino acids 9 to 242 (234 residues), 87.5 bits, E=4.6e-29

Best Hits

KEGG orthology group: None (inferred from 97% identity to kpn:KPN_01695)

MetaCyc: 42% identical to propanoyl-CoA transferase monomer (Megasphaera elsdenii)
Propionate CoA-transferase. [EC: 2.8.3.1]; 2.8.3.1 [EC: 2.8.3.1]; 2.8.3.1 [EC: 2.8.3.1]; 2.8.3.1 [EC: 2.8.3.1]

Predicted SEED Role

"Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 2.8.3.8)

MetaCyc Pathways

• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (7/9 steps found)
• acetyl-CoA fermentation to butanoate (5/7 steps found)
• 1,2-propanediol biosynthesis from lactate (engineered) (4/6 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to acetone (3/5 steps found)
• (R)-lactate fermentation to propanoate II (acrylate pathway) (2/4 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• 4-aminobutanoate degradation V (3/7 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (9/17 steps found)
• succinate fermentation to butanoate (1/7 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism
• Styrene degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.3.1 or 2.8.3.8

Sequence Analysis Tools

See A0A285B8Z7 at UniProt or InterPro

Protein Sequence (533 amino acids)

>BWI76_RS23700 glycerate CoA-transferase (Klebsiella michiganensis M5al)
MSSKFIDAHQAAQWVASGDTVCTVGMTLIGAAESILSAIEARFLTAGEPRDLTLLHAAGQ
SDRQRGIQHFAHPGMVTRLIGSHWGLAPRWMAMINNNEVEAWCLPQGQIVHLYSAMAAGL
AGRLSPVGLGTFVDPRMEGGRMNARTRERPDLIEHVTFRGAEYLFYPAIPLDVVIVRGTH
ADEDGNLTTDEEVMKLEVLHAVLAARRYGAKVLAQVKYRVAKGSLHPKSVTVPGNLIDAI
VVCEEPQMDHRQTSSWDFDPALCGDIQLPAAQSAPLPLDLRKLIGRIACRYLTPGCVINL
GTGIPNDVIGAIIHEERLGEQVTITVESGIYGGQQAGGVDFGIGRNLSAMIGHQDQMLYY
NGAGVDITFMGAGEMDPHGHVNATRLGASCPGAGGFIDITQNARHVVFCSSFTAKGLEIA
CEQGALHIRREGEVRKFVAGVNQISYNGELARAKGQTMHYVTERAVFELRPEGPVLTEIA
PGIDLERDILAHMDFRPTIAADLQVMDSRLFTPQPCGLAEHLSRNSSSDSQGN