Protein Info for BWI76_RS23630 in Klebsiella michiganensis M5al
Annotation: acetoin(diacetyl) reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to BUDC_KLEPN: Diacetyl reductase [(S)-acetoin forming] (budC) from Klebsiella pneumoniae
KEGG orthology group: K03366, (R,R)-butanediol dehydrogenase / diacetyl reductase [EC: 1.1.1.303 1.1.1.4] (inferred from 80% identity to ilo:IL0792)MetaCyc: 56% identical to acetoin reductase subunit (Klebsiella pneumoniae)
RXN-11032 [EC: 1.1.1.304]; 1.1.1.- [EC: 1.1.1.304]
Predicted SEED Role
"2,3-butanediol dehydrogenase, S-alcohol forming, (S)-acetoin-specific (EC 1.1.1.76)" in subsystem Acetoin, butanediol metabolism (EC 1.1.1.76)
MetaCyc Pathways
- superpathway of (R,R)-butanediol biosynthesis (5/5 steps found)
- pyruvate fermentation to (R)-acetoin I (3/3 steps found)
- pyruvate fermentation to (S)-acetoin (3/3 steps found)
- superpathway of 2,3-butanediol biosynthesis (5/6 steps found)
- (R,R)-butanediol biosynthesis (1/1 steps found)
- (S,S)-butanediol biosynthesis (1/1 steps found)
- (R,R)-butanediol degradation (1/1 steps found)
- (S,S)-butanediol degradation (1/1 steps found)
- meso-butanediol biosynthesis I (1/1 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.303, 1.1.1.4
Use Curated BLAST to search for 1.1.1.303 or 1.1.1.304 or 1.1.1.4 or 1.1.1.76
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B903 at UniProt or InterPro
Protein Sequence (259 amino acids)
>BWI76_RS23630 acetoin(diacetyl) reductase (Klebsiella michiganensis M5al) MAIENKVALVTGAGQGIGRGIALRLAKDGASLMLVDVNPEGIAAVAAEVEALGRKAATFV ANIADRAQVYAAIDEAEKQLGGFDIIVNNAGIAQVQALADVTPEEVDRIMRINVQGTLWG IQAAAKKFIDRQQKGKIINACSIAGHDGFALLGVYSATKFAVRALTQAAAKEYASRGITV NAYCPGIVGTGMWTEIDKRFAEITGAPVGETYKKYVEGIALGRAETPDDVASLVSYLAGP DSDYVTGQSILIDGGIVYR