Protein Info for BWI76_RS23620 in Klebsiella michiganensis M5al

Annotation: multidrug resistance transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 492 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 18 to 478 (461 residues), 271.3 bits, see alignment E=7.7e-85 PF02321: OEP" amino acids 71 to 269 (199 residues), 75 bits, see alignment E=3.6e-25 amino acids 298 to 448 (151 residues), 53.3 bits, see alignment E=1.6e-18

Best Hits

Swiss-Prot: 49% identical to MDTP_ECOLI: Multidrug resistance outer membrane protein MdtP (mdtP) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 89% identity to eae:EAE_02475)

MetaCyc: 49% identical to putative multidrug efflux pump outer membrane channel (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-351

Predicted SEED Role

"Outer membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B9A5 at UniProt or InterPro

Protein Sequence (492 amino acids)

>BWI76_RS23620 multidrug resistance transporter (Klebsiella michiganensis M5al)
MIKITPPPRIWRTSALAFALTLLTGCALIQDDPGQVPLVNPQQAELAQVIHLANSGWPTA
RWWEGYQDPQLNMLVNRALQNSPTMQAARLRISQSQSTVELAQSAMGVQATAVAAQNRLR
VTNKQFTWPYSFSLPVDKNGPWYTLNTVGVGATLNIDLWGADRARVAAAIGEKNARQAET
AGIELDLASSVAQLYFAMQATFQKIELLNQLEAIASLSVQAHEHRTARGVEDSVDVANAQ
DELLAAQQQVITAQGTITQYRETLRALIGADAHSMPEIHPVALPALQETLPDSLSFELLA
RRPDLQALRGYVSASMSQVDAAKAAFYPHFDIKAFWGYNALSVGDLFKSSFQQINILPGL
YLPLFDGGRLNATLKSTRTASNILIKQYNQAVLDAVRDVAISSSQLNDLNQQAALQQQKV
TAAMVTTRSASAHHQRGLVSYYAAQEARRPAIAQQLLLLDIQVQRLSTDITLIKALGGDY
RGPALSENAPLR