Protein Info for BWI76_RS23235 in Klebsiella michiganensis M5al

Annotation: pitrilysin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 961 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00675: Peptidase_M16" amino acids 54 to 191 (138 residues), 132.8 bits, see alignment E=1.4e-42 PF05193: Peptidase_M16_C" amino acids 215 to 393 (179 residues), 75.5 bits, see alignment E=7.6e-25 amino acids 682 to 857 (176 residues), 60.1 bits, see alignment E=4.2e-20 PF16187: Peptidase_M16_M" amino acids 400 to 675 (276 residues), 190.1 bits, see alignment E=7.1e-60

Best Hits

Swiss-Prot: 84% identical to PTRA_ECOL6: Protease 3 (ptrA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 92% identity to eae:EAE_02105)

MetaCyc: 84% identical to protease 3 (Escherichia coli K-12 substr. MG1655)
Pitrilysin. [EC: 3.4.24.55]

Predicted SEED Role

"Protease III precursor (EC 3.4.24.55)" (EC 3.4.24.55)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B827 at UniProt or InterPro

Protein Sequence (961 amino acids)

>BWI76_RS23235 pitrilysin (Klebsiella michiganensis M5al)
MPRSLWFKVIVLLAALWAPLSQADTGWQPIQETIRKSDKDTRQYQAIRLDNGMVVLLVSD
PQAVKSLSALVVPVGSLQDPAEHQGLAHFLEHMTLMGSKKYPQPDSLAEFLKMHGGSHNA
STAPYRTAFYLEVENDALDGAVDRLADAIAAPLLDKKYADRERNAVNAELTMARTRDGMR
MAQVSAETINPAHPGSQFSGGNLETLSDKPGKPVLDALHAFRDSWYSANLMKAVIYSNKP
LPELASIAAATYGRVPNHDISKPEITVPVVTDAQKGIIIHYVPAMPRKVLRVEFRIDNNS
DKFRSKTDELVTYMIGNRSPGTLSDWLQKQGLVEGIRADSDPVVNGNSGVLAISATLTDK
GLAHRDEVTAAIFSYLNLLRSQGIDKRYFDELAHVLALDFRYPSINRNMDYVEWLADTMI
RVPVEHTLDVVNIADRYDPQAIKDRLAMMTPQNARIWYISPKEPHNKTAYFVNAPYQVDK
ISEQTFADWQQKSSAIDLKLPVLNPYIPDDFSLIKSDKAYPHPQLIVDEPTLRVIYAPSQ
YFASEPKADISLVLRNPQAMDSARRQVIFALNDYLAGIALDQLSNQAAVGGISFSTGANN
GLMVNANGYTQHLPELFNALLDGYFSYTPTEEQLEQAKSWYAQMMDSADKGKAYDQAIMP
IQMVSQVPYFQREERRALLPSIALKEVLEYRANLKAKGRPELMVIGNLTADQSTAMARQI
QKQLGADGNEWCRNKDVLVDRKQLAIFEKAGNSTDSALAAVFAPPNVDEYASSAASSLLG
QIVQPWFYNQLRTEEQLGYAVFAFSMNVGRQWGMGFLLQSNDKQPAYLWQRFQAFFPTAE
AKLRAMKPEEFAQIQQAVIGQMLEAPQTLGDEASKLSKDFDRGNMRFDSRDKVVAQIKLL
TPQKLADFFHQTVVDPQGMAILSQVSGSQNGKAEYAHPQDGKVWENVSALQKSLPLMREN
E