Protein Info for BWI76_RS23230 in Klebsiella michiganensis M5al

Annotation: exodeoxyribonuclease V subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1181 TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 8 to 1172 (1165 residues), 1404.9 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 15 to 436 (422 residues), 171.7 bits, see alignment E=6.7e-54 PF13245: AAA_19" amino acids 374 to 423 (50 residues), 27 bits, see alignment (E = 1.1e-09) PF13361: UvrD_C" amino acids 550 to 809 (260 residues), 58.9 bits, see alignment E=1.5e-19

Best Hits

Swiss-Prot: 76% identical to RECB_ECOLI: RecBCD enzyme subunit RecB (recB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 90% identity to eae:EAE_02100)

MetaCyc: 76% identical to exodeoxyribonuclease V subunit RecB (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]; Exodeoxyribonuclease V. [EC: 5.6.2.4, 3.1.11.5]; RXN-19004 [EC: 5.6.2.4, 3.1.11.5]

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7Y7 at UniProt or InterPro

Protein Sequence (1181 amino acids)

>BWI76_RS23230 exodeoxyribonuclease V subunit beta (Klebsiella michiganensis M5al)
MTDTAESLDPLRLPLTGERLIEASAGTGKTFTIAALYLRLLLGLGGESAFPRQVSVEELL
VVTFTEAATEELRGRIRSNIHELRIACLRDSADNSLYAGLLAEIADKTQAAQTLLLAERQ
MDEAAVFTIHGFCQRMLSLNAFESGMLFEQQLIEDESRLRYQACADFWRRHCYPLPREIA
AVIHEAWKGPRDLLKSIDRWLQGEAPQLKSPPSADETLAERHQQIIERINALKQQWLAQV
GEVEAVLENSGLDRRKFNRGNQGKWLEKVTAWAEEETLSYQLPDALEKFSQAFLLERTKA
DGAPPVHPLFSAVEALLASPLTLTDLVIARAMVEIREAVAREKRRRGELGFDDMLSRLDD
ALRGESGEALASAIRQRFPVAMIDEFQDTDPQQYRIFRRIWRRQADTALLLIGDPKQAIY
AFRGADIFTYMKARGDVTAHYTLDTNWRSAPGMVDSVNRLFSLSDNPFMFREIPFMPVKS
ADKNQGLRFTVDDAAIPAMNIWLMSGEAVGAGDYQTFMAQLCAAQIRDWLSAGQQGKALL
WRGEKAEPVRASDITVLVRNRQEASLIRDALQLLSIPSVYLSNRDSVFETPEAQELLWLL
QAVLAPERENTLRSALATSMFGLNALDIENLNHDERAWDELVEEFSRYRQLWRQRGVMPM
LRALMSARRIAENLLATNGGERRLTDILHISELLQEAANQLESEHALVRWLAQHIAEPDS
NASSQQMRLESDKHLVQIVTIHKSKGLEYPLVWLPFIARFRKQDQAFYHDRSSFAAVLDL
GQDEGSMALAEAERLAEDLRLLYVALTRAVWHCSLGVAPLSTRRSDKPGASDFHHSALGR
LLQDGEAMDAAGLMARLQAFCSGDIALQLPDMAAQTPWQAPQAALPPLSARMLERSVTDD
WRVTSYSGLQQNGHSAAQDLLPRLDVDAAGVGEVVEEPQLTPHQFPRGASPGTFLHSLFE
DLDFTQPVPADWMAGKLQLSGFEEKWTPVLTAWLDGVLRTPLPGAEIALNQLSARDKQVE
MAFYLPIEQPLDPLRLDALIRQYDPLSVDTPTLDFRQVRGMLKGFIDLVFRHNGRYYLLD
YKSNWLGENREAYTQEAMRQAMRSHRYDLQYQLYSLALHRYLRHRIADYDYERHFGGVIY
LFLRGMDGQDGGQGIFTTRPALPLIDGLDALFAGETQEVVL