Protein Info for BWI76_RS22900 in Klebsiella michiganensis M5al

Annotation: alkanesulfonate monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 TIGR03565: alkanesulfonate monooxygenase, FMNH(2)-dependent" amino acids 10 to 355 (346 residues), 612.4 bits, see alignment E=1.1e-188 PF00296: Bac_luciferase" amino acids 10 to 331 (322 residues), 285.2 bits, see alignment E=3.8e-89

Best Hits

Swiss-Prot: 71% identical to SSUD1_RHILO: Alkanesulfonate monooxygenase 1 (ssuD1) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K04091, alkanesulfonate monooxygenase [EC: 1.14.14.5] (inferred from 92% identity to kpn:KPN_03147)

MetaCyc: 63% identical to alkanesulfonate monooxygenase subunit (Pseudomonas putida DS1)
RXN-18086 [EC: 1.14.14.34]

Predicted SEED Role

"Alkanesulfonate monooxygenase (EC 1.14.14.5)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.14.14.5)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.14.14.5

Use Curated BLAST to search for 1.14.14.34 or 1.14.14.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXQ7 at UniProt or InterPro

Protein Sequence (390 amino acids)

>BWI76_RS22900 alkanesulfonate monooxygenase (Klebsiella michiganensis M5al)
MTQKADDKINVFWFLPTHGDGRYLGTAEGGRPVDLPYLQQVALAADSLGYYGVLIPTGKS
CEDSWLVASALAPLTRRLRYLVAVRPGLQPPTLAARMATTLDRLSGGRLLINVVTGGDPV
ENKGDGIFLSHGERYQVTREFLDVYSRLLKGEKVDYHGEHIHVEGAEVLFPPVQENGPPL
FFGGSSEEAIDVAADQIDTYLTWGEPPALVAEKLAVVRERAAARGRTLQYGIRLHVIVRE
TEEEAWAAADRLISHLDDETIAQAQKIFARMDSTGQARMSSLHKGSRDNLRIAPNLWAGV
GLVRGGAGTALVGNPQQVAERIREYQALGITNFIFSGYPHLEEAHRFADLVMPLLPLAGH
DQPKARSVNTGPFGETIGGDKRPVKQVSAS