Protein Info for BWI76_RS22865 in Klebsiella michiganensis M5al

Annotation: NADPH-dependent 7-cyano-7-deazaguanine reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 TIGR03138: queuine synthase" amino acids 11 to 281 (271 residues), 425.8 bits, see alignment E=4.2e-132 PF14819: QueF_N" amino acids 21 to 131 (111 residues), 158.1 bits, see alignment E=9.5e-51 PF14489: QueF" amino acids 195 to 269 (75 residues), 84.3 bits, see alignment E=5.5e-28

Best Hits

Swiss-Prot: 92% identical to QUEF_KLEP3: NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_02025)

MetaCyc: 85% identical to 7-cyano-7-deazaguanine reductase (Escherichia coli K-12 substr. MG1655)
PreQ(1) synthase. [EC: 1.7.1.13]

Predicted SEED Role

"NADPH dependent preQ0 reductase (EC 1.7.1.13)" (EC 1.7.1.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXV2 at UniProt or InterPro

Protein Sequence (281 amino acids)

>BWI76_RS22865 NADPH-dependent 7-cyano-7-deazaguanine reductase (Klebsiella michiganensis M5al)
MSSYDNHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLDLHADNLPFHGADIWTLY
ELSWLNAKGLPQVAVGHVELSDTTVNLVESKSFKLYLNSFNQTRFADWQAVEATLARDLS
ACAQGEVKVSLYRLDELEGQPVAHLHGTCIDDQDIEIDNYQFSADYLQDAACGKVVEETL
VSHLLKSNCLITHQPDWGSVQIQYRGAKIDREKLLRYLVSFRHHNEFHEQCVERIFNDIQ
RFCQPEALSVYARYTRRGGLDINPWRSNTDFVPATGRLARQ