Protein Info for BWI76_RS22830 in Klebsiella michiganensis M5al
Annotation: glucarate dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 95% identical to GUDX_ECOLI: Glucarate dehydratase-related protein (gudX) from Escherichia coli (strain K12)
KEGG orthology group: K13918, glucarate dehydratase-related protein (inferred from 95% identity to ecc:c3352)MetaCyc: 64% identical to D-glucarate dehydratase subunit (Acinetobacter baylyi ADP1)
Glucarate dehydratase. [EC: 4.2.1.40]
Predicted SEED Role
"Glucarate dehydratase (EC 4.2.1.40)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.40)
MetaCyc Pathways
- superpathway of D-glucarate and D-galactarate degradation (5/5 steps found)
- D-glucarate degradation I (4/4 steps found)
- D-glucarate degradation II (1/3 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (19/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.40
Use Curated BLAST to search for 4.2.1.40
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AXS2 at UniProt or InterPro
Protein Sequence (446 amino acids)
>BWI76_RS22830 glucarate dehydratase (Klebsiella michiganensis M5al) MTTQSSPVITDMKVIPVAGQDSMLLNIGGAHNAYFTRNIVVLTDSAGNTGVGEAPGGEVI YQTLVDAIPMVLGQEIARLNKVVQQVHKGNQAADFDTFGKGAWTFELRVNAVAALEAALL DLLGKALNVPVCELLGPGKQRDAVTVLGYLFYVGDRTKTDLPYLESTPGSHAWYRLRHQQ ALNSEAVVRLAEAAQDRYGFKDFKLKGGVLPGEQEIETARALKKRFPDARITVDPNGAWL LDEAIALCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHA VMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGN PTAIDTHWIWQEGDCRLTKNPLEIKHGKIAVPDAPGLGVELDWEQVQKAHEAYTRLPGGA RNDAGPMQYLIPGWTFDRKRPVFGRH