Protein Info for BWI76_RS22800 in Klebsiella michiganensis M5al

Annotation: nucleoside triphosphate pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00444: MazG family protein
  • amino acids 10 to 257 (248 residues), 397.2 bits, E=1.6e-123
• PF03819: MazG
  • amino acids 27 to 100 (74 residues), 98.6 bits, E=2e-32
  • amino acids 168 to 227 (60 residues), 29.8 bits, E=6.1e-11

Best Hits

Swiss-Prot: 89% identical to MAZG_ECOLI: Nucleoside triphosphate pyrophosphohydrolase (mazG) from Escherichia coli (strain K12)

KEGG orthology group: K02428, nucleoside-triphosphate pyrophosphatase [EC: 3.6.1.19] (inferred from 96% identity to kpn:KPN_03125)

MetaCyc: 89% identical to nucleoside triphosphate pyrophosphohydrolase (Escherichia coli K-12 substr. MG1655)
Nucleotide diphosphatase. [EC: 3.6.1.9]

Predicted SEED Role

"Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8)" (EC 3.6.1.8)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides dephosphorylation (3/3 steps found)
• dZTP biosynthesis (3/5 steps found)
• tunicamycin biosynthesis (2/9 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Nicotinate and nicotinamide metabolism
• Pantothenate and CoA biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Riboflavin metabolism
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.19 or 3.6.1.8 or 3.6.1.9

Sequence Analysis Tools

See A0A285AXT2 at UniProt or InterPro

Protein Sequence (263 amino acids)

>BWI76_RS22800 nucleoside triphosphate pyrophosphohydrolase (Klebsiella michiganensis M5al)
MSQIDRLLGIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIAREDFDDLRGELG
DLLFQVVFYAQMAQEEGRFDFNDICAAIGDKLERRHPHIFGDASAGNSSEVLARWEQIKS
AERAEKSQHSALDDIPHSLPALMRAHKIQKRCSAVGFDWTSLGPVLDKVHEEIDEVMHEA
QQAVVDEAKLEEEVGDLLFATVNLSRHLGVKAEVALQKANLKFERRFREVERIVAARGLE
MTGVDLDTMELVWQEVKRQETDL