Protein Info for BWI76_RS22750 in Klebsiella michiganensis M5al

Annotation: CRISPR-associated helicase/endonuclease Cas3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 886 transmembrane" amino acids 201 to 219 (19 residues), see Phobius details PF18019: Cas3_HD" amino acids 5 to 221 (217 residues), 119.5 bits, see alignment E=2.2e-38 TIGR01596: CRISPR-associated endonuclease Cas3-HD" amino acids 22 to 217 (196 residues), 81.6 bits, see alignment E=7.4e-27 TIGR01587: CRISPR-associated helicase Cas3" amino acids 291 to 661 (371 residues), 209.4 bits, see alignment E=8.3e-66

Best Hits

KEGG orthology group: K07012, (no description) (inferred from 75% identity to sea:SeAg_B3072)

Predicted SEED Role

"CRISPR-associated helicase Cas3" in subsystem CRISPRs

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXR5 at UniProt or InterPro

Protein Sequence (886 amino acids)

>BWI76_RS22750 CRISPR-associated helicase/endonuclease Cas3 (Klebsiella michiganensis M5al)
MFNYWGKSLRGDDDGGDDYHLLCWHSLDVAAVGYWMVQDNIHSINDYLNKLGLHHREQAA
QFFAWLLCWHDIGKFAHSFQQLYRHEELNAQDEYIKHYEAIPHATLGYWLWNAFLSECSE
LFPESSLSARKLKRAIALWMPLTTGHHGRPPSSVTELKNFRQQDKEAARDFLIRVKSLFP
LIEVPDFWDDDDGITLFKHLSWFISAAIVLADWVGSSIRYFPRIAKPMGVEEYWQQHALV
KAKVAITVFPPSSGVAPFSGIKALFPFIQQPTPLQKKALELDIDKEGPQLFILEDVTGAG
KTEAALILTHRLMATGKAQGLYFGLPTMATANAMFDRMLESWLGLYQPGSRPSLVLAHSA
RGLMDRFTQSLWSGDLSGSEEPDDLLPSSQGCAAWFADSNKKALLAEVGVGTLDQAMMAV
MPFKHSNLRMLGLSHKILLADEIHAYDSYMSRILECLVEKQASDGNTTILLSATLSQQQR
DRLVAAFAKGAQGRVEAPLLGHDDYPWLTQVTGSKVVSQYVETRKEVERSVKVNWTHGEQ
ECLERIEQAIGQGKCIAWIRNSVDDAIRIYRQLLTRGVVPSESLLLFHSRFAFYDRQRIE
NRALAQFGKQGVAKRCGQVLIATQVIEQSLDIDVDEMISDLAPVDLLIQRAGRLQRHIRD
RNGLLKASGRDERNPPELLIFAPEWDDEPQEDWFSNAMRNSAYVYPDHGRLWLTQRVLRE
QGEIRMPQSARLLIESVYGEEVDMPPGFVKTEQLQEGKFYCDRAFAKQNIINFAPGYSPE
CSDYLPEKLSTRLAEESVSLWLAKIVDGAVMPYAPGEHAWEMSALRVRQGWWDKHRGDFV
LLDGKRLQQWCAEQHQNQEFALVIVVSDLETCGYSAHEGLIGKMEV