Protein Info for BWI76_RS22720 in Klebsiella michiganensis M5al

Annotation: subtype I-E CRISPR-associated endonuclease Cas1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 TIGR03638: CRISPR-associated endonuclease Cas1, subtype I-E/ECOLI" amino acids 8 to 272 (265 residues), 379.6 bits, see alignment E=7.5e-118 TIGR00287: CRISPR-associated endonuclease Cas1" amino acids 16 to 128 (113 residues), 77.3 bits, see alignment E=1.1e-25 amino acids 133 to 256 (124 residues), 99.9 bits, see alignment E=1.5e-32 PF01867: Cas_Cas1" amino acids 132 to 225 (94 residues), 32.4 bits, see alignment E=2.8e-12

Best Hits

Swiss-Prot: 84% identical to CAS1_ECOLI: CRISPR-associated endonuclease Cas1 (ygbT) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 94% identity to sea:SeAg_B3066)

Predicted SEED Role

"CRISPR-associated protein Cas1" in subsystem CRISPRs

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXL9 at UniProt or InterPro

Protein Sequence (306 amino acids)

>BWI76_RS22720 subtype I-E CRISPR-associated endonuclease Cas1 (Klebsiella michiganensis M5al)
MTFVPLSPIPLKDRTSMIFLQYGQVDVLDGAFVLIDKTGIRTHIPVGSVACIMLEPGTRV
SHAAVRLASTVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQAKLALDDDLRLKVVRKMY
ELRFREPPPARRSVEQLRGIEGARVRQTYLLLAKQYGVKWHGRNYDPKDWEKGDVVNRCI
SAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYDIADIIKFEAVVPKAFEIAARAP
AEPDREVRLACRDIFRSTKLTGKLIPLIEDVLAAGEIEPPKPAPDMLPPAIPEPQRLGDG
GHRGHG