Protein Info for BWI76_RS22535 in Klebsiella michiganensis M5al

Annotation: membrane fusion protein of a type I secretion system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 43 to 379 (337 residues), 130.3 bits, see alignment E=3.9e-42 PF16576: HlyD_D23" amino acids 64 to 268 (205 residues), 56.9 bits, see alignment E=3.8e-19 PF13533: Biotin_lipoyl_2" amino acids 64 to 112 (49 residues), 39.8 bits, see alignment 6e-14 PF13437: HlyD_3" amino acids 189 to 272 (84 residues), 37 bits, see alignment E=9.4e-13

Best Hits

KEGG orthology group: K13888, macrolide-specific efflux protein MacA (inferred from 67% identity to cro:ROD_35081)

Predicted SEED Role

"Macrolide-specific efflux protein MacA" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXL3 at UniProt or InterPro

Protein Sequence (386 amino acids)

>BWI76_RS22535 membrane fusion protein of a type I secretion system (Klebsiella michiganensis M5al)
MRRQFLRRRKAWLLALVAILATIAAVFYQRSLPAQAESLYAVALERGDIEKVVLASGIIK
PAVQVSVGAQVSGQLRKLYVRQGERVRKGQLLAEIDPTLQLSDLRNATAQLASARAQKLA
SEATLVQYRKALARERAMNRDGSGVSSDLEQAQAQYAAQVQQIAVNEAQIVQAEMSVQTA
NANLGYTRILAPMDGEVLGIVTREGQTIVSSQIAPTILVLADLDKMQVQTRISEADIQKI
HPGQPLWFYVIADPQTRYEGELGYVQPAPQEALEEQNAGASAGNQQSSAIYYNGTFEVDN
RQRLLKTSMTAQVFIRIAQVKDVLRVPVAALGRSLGDERYIVMVQTASGSEERTVRIGIN
DRQYAQVLEGLRPGERVVIPQDAGSV