Protein Info for BWI76_RS22330 in Klebsiella michiganensis M5al

Annotation: formate hydrogenlyase subunit 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00361: Proton_antipo_M
  • amino acids 150 to 404 (255 residues), 129.1 bits, E=1e-41

Best Hits

Swiss-Prot: 72% identical to HYCC_ECOLI: Formate hydrogenlyase subunit 3 (hycC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 90% identity to kva:Kvar_1006)

MetaCyc: 72% identical to hydrogenase 3 membrane subunit HycC (Escherichia coli K-12 substr. MG1655)
Ferredoxin hydrogenase. [EC: 1.12.7.2]; FHLMULTI-RXN [EC: 1.12.7.2]

Predicted SEED Role

"Formate hydrogenlyase subunit 3" in subsystem Formate hydrogenase

MetaCyc Pathways

• mixed acid fermentation (16/16 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (9/9 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• hydrogen production III (1/1 steps found)
• hydrogen production V (1/1 steps found)
• hydrogen production VIII (1/1 steps found)
• hydrogen production VI (1/2 steps found)
• superpathway of hydrogen production (1/2 steps found)
• superpathway of photosynthetic hydrogen production (3/6 steps found)
• L-glutamate degradation VII (to butanoate) (7/12 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.12.7.2

Sequence Analysis Tools

See A0A285AXE2 at UniProt or InterPro

Protein Sequence (608 amino acids)

>BWI76_RS22330 formate hydrogenlyase subunit 3 (Klebsiella michiganensis M5al)
MSVISLVNQAVAWYAASAVLAFLFALRKPLSGAIAGIGGAVASAMLVLAGGTALLMPERV
SGGMLQMLHLTLRVGGVNALWLLAIGLSALPISLFNIAWHRHSQVKANGLLVNLLLAAAV
CAVVVTNFGALVVMAEIMALCAAFLTGCAQSGKLWFALGRLGTLLMAWSCWLVWSSYGTL
ELAQINQLALAVPQNALLWLPGLIGFALLAGVIPLHGWAPQAHAGASAPAAALFSTVVMK
VGLYGILTVSLAGSVPPLWWGVMLLVLGMITAFVGGLYALMEHNIQRLLAYHTLENIGII
LLGLGAFVTGVATHNPTLMVLGFIGGMYHLINHSLFKTTLFLGAGAVWFRTGHRDIEKLG
GIGKKMPLISLSMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSTGDAFVGRLLGPLLAVG
LAITGALAVMCMAKVYGVTFLGAPRSKEAENATCAPGLMTFSVVLSAVCCVIGGVAAPWL
LPLVSGAFPVQAEVSSVVSQPLIAILLIACPLLPLLMMIFFKGDRLPARSRGAAWVCGYD
HEKSMVVTAHGFAMPVKEAFAPILKLRHWLNPVRLVPGWQNASAPALFRGVALVELAVLV
VIVISRGA