Protein Info for BWI76_RS22315 in Klebsiella michiganensis M5al

Annotation: formate hydrogenlyase complex iron-sulfur subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12797: Fer4_2
  • amino acids 32 to 52 (21 residues), 25.7 bits, see alignment (E = 2.4e-09)
• PF12837: Fer4_6
  • amino acids 32 to 55 (24 residues), 29.4 bits, see alignment (E = 1.6e-10)
• PF13237: Fer4_10
  • amino acids 33 to 86 (54 residues), 36.6 bits, E=1e-12
• PF00037: Fer4
  • amino acids 34 to 56 (23 residues), 29.8 bits, see alignment (E = 1.1e-10)
  • amino acids 70 to 91 (22 residues), 35 bits, see alignment (E = 2.5e-12)
• PF12838: Fer4_7
  • amino acids 39 to 89 (51 residues), 49.6 bits, E=1.4e-16
• PF13187: Fer4_9
  • amino acids 39 to 90 (52 residues), 37.2 bits, E=7.5e-13

Best Hits

Swiss-Prot: 91% identical to HYCF_ECOLI: Formate hydrogenlyase subunit 6 (hycF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 98% identity to eae:EAE_01650)

MetaCyc: 91% identical to hydrogenase 3 iron-sulfur protein HycF (Escherichia coli K-12 substr. MG1655)
Ferredoxin hydrogenase. [EC: 1.12.7.2]

Predicted SEED Role

"Formate hydrogenlyase complex 3 iron-sulfur protein; Formate hydrogenlyase subunit 6; Ni,Fe-hydrogenase III medium subunit"

MetaCyc Pathways

• superpathway of fermentation (Chlamydomonas reinhardtii) (9/9 steps found)
• hydrogen production III (1/1 steps found)
• hydrogen production VIII (1/1 steps found)
• hydrogen production VI (1/2 steps found)
• superpathway of hydrogen production (1/2 steps found)
• superpathway of photosynthetic hydrogen production (3/6 steps found)
• L-glutamate degradation VII (to butanoate) (7/12 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.12.7.2

Sequence Analysis Tools

See A0A285AXH6 at UniProt or InterPro

Protein Sequence (180 amino acids)

>BWI76_RS22315 formate hydrogenlyase complex iron-sulfur subunit (Klebsiella michiganensis M5al)
MFTFIKKVIKTGTATHSYPLEPMPVDKNFRGKPEHNPQQCIGCAACVNACPSNALTVETD
LAANQLGWQFNLGRCIFCGRCEEVCPTAAIRLSQEYELAVWKKEDFLQQSRFAICHCRVC
ERPFAVQKEIDYAIALLKHNGDSRAELHRESFETCPECKRQKCLVPSDRIELTRHMREAS