Protein Info for BWI76_RS22290 in Klebsiella michiganensis M5al

Annotation: PTS cellobiose/arbutin/salicin transporter subunit IIBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00367: PTS_EIIB
  • amino acids 10 to 43 (34 residues), 58.3 bits, see alignment (E = 3.9e-20)
• PF02378: PTS_EIIC
  • amino acids 113 to 396 (284 residues), 144.6 bits, E=3.8e-46

Best Hits

Swiss-Prot: 89% identical to PTIBC_ECOLI: PTS system arbutin-, cellobiose-, and salicin-specific EIIBC component (ascF) from Escherichia coli (strain K12)

KEGG orthology group: K02752, PTS system, arbutin-, cellobiose-, and salicin-specific IIB component [EC: 2.7.1.69] K02753, PTS system, arbutin-, cellobiose-, and salicin-specific IIC component (inferred from 95% identity to kva:Kvar_1015)

MetaCyc: 89% identical to beta-glucoside specific PTS enzyme IIBC component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-153A [EC: 2.7.1.199]

Predicted SEED Role

"PTS system, arbutin-, cellobiose-, and salicin-specific IIBC component (EC 2.7.1.69)" in subsystem Beta-Glucoside Metabolism (EC 2.7.1.69)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
• glycolysis I (from glucose 6-phosphate) (13/13 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.199 or 2.7.1.69

Sequence Analysis Tools

See A0A285AXG5 at UniProt or InterPro

Protein Sequence (480 amino acids)

>BWI76_RS22290 PTS cellobiose/arbutin/salicin transporter subunit IIBC (Klebsiella michiganensis M5al)
MSKNYAALAQQVVAAIGGADNVAAVTHCMTRLRFVVKDNERVDSATLKGLKGVLGVVRSD
NQCQVIIGNTVSQAYREVVALLPGDLRPVEPQGKPPITLKRIGAGILDALIGTMSPLIPA
IIGGSMVKLLAMILEMTGALPKGAPTLTILTVIGDGAFFFLPLMVAASAAVKFKTNMSLA
IAIAGVLVHPSFIELMAKAAQGEHVEFAFIPVTAVKYTYTVIPALVMTWCLSYIERWVDR
ITPAVTKNFLKPMLIVLIAAPLSILLIGPLGIWIGSAISALVYTIHSYLGWLSVAIMGAL
WPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNPELRQT
ALAAAASAILAGISEPALYGVAVRLKRPLIASLISGFICGAVAGIAGLASHSMAAPGLFT
SVQFFDPANPMTIVWVFGVMALAVVLSFVLTLMLGFEDIPVEEAAADARQRQAGAQASHA