Protein Info for BWI76_RS22170 in Klebsiella michiganensis M5al

Annotation: alanine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 7 to 555 (549 residues), 790.8 bits, E=1e-241
• TIGR00344: alanine--tRNA ligase
  • amino acids 7 to 854 (848 residues), 1176.1 bits, E=0
• PF07973: tRNA_SAD
  • amino acids 653 to 696 (44 residues), 62.9 bits, 3.4e-21
• PF02272: DHHA1
  • amino acids 733 to 871 (139 residues), 116.2 bits, E=2.4e-37

Best Hits

Swiss-Prot: 92% identical to SYA_CITK8: Alanine--tRNA ligase (alaS) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: None (inferred from 96% identity to eae:EAE_01510)

MetaCyc: 91% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• tRNA charging (21/21 steps found)
• enterobactin biosynthesis (7/11 steps found)
• bacillibactin biosynthesis (6/12 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (3/8 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.96 or 6.1.1.7

Sequence Analysis Tools

See A0A285AXB2 at UniProt or InterPro

Protein Sequence (875 amino acids)

>BWI76_RS22170 alanine--tRNA ligase (Klebsiella michiganensis M5al)
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYS
RATTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQDAIKYAWELLTGESW
FALPKEKLWVTVYETDDEAFDIWANEVGVPRERIIRIGDNKGAPFASDNFWQMGDTGPCG
PCTEIFFDHGDHIWGGPPGTPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
LERIAAVLQHVNSNYDIDLFRDLIKSVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVI
PSNENRGYVLRRIIRRAIRHGNMLGAKETFFWKLVAPLIDVMGSAGEELKQQQAQVEQVL
KTEEEQFARTLERGLALLDEELAKLQGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKV
DEAGFEAAMEEQRRRARESSGFGADYNAMIRVDGVSEFKGYDSLELNGKVTALFIDGKAV
DAVNAGQEAVVILDQTPFYAESGGQVGDKGELKGAGFSFQVNDAQKYGQAIGHIGKVTGG
SLKVGDAVQADVDEARRQRIRLNHSATHLMHAALRQVLGTHVAQKGSLVNDKALRFDFSH
FEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDIDAARESGAMALFGEKYDDRVRVLSMGD
FSTELCGGTHASRTGDIGLFRITSESGTAAGVRRIEAVTGEGAMAMLHAQSDQLSDIAQL
LKGDTHSLGDKVRAALERSRQLEKELQQLKEQAAVQESANLSSKAEEINGVKLLVSELAG
VEPKMLRTMVDDLKNQLGSTVVVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVG
GKGGGRPDMAQAGGTDASALPAALASVKGWVSAKL