Protein Info for BWI76_RS22120 in Klebsiella michiganensis M5al

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 transmembrane" amino acids 191 to 209 (19 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 6 to 517 (512 residues), 899.5 bits, see alignment E=3e-275 PF04262: Glu_cys_ligase" amino acids 10 to 380 (371 residues), 564.4 bits, see alignment E=4.3e-174

Best Hits

Swiss-Prot: 94% identical to GSH1_KLEP3: Glutamate--cysteine ligase (gshA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 93% identity to kpn:KPN_03019)

MetaCyc: 88% identical to glutamate--cysteine ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--cysteine ligase. [EC: 6.3.2.2]

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXH1 at UniProt or InterPro

Protein Sequence (518 amino acids)

>BWI76_RS22120 glutamate--cysteine ligase (Klebsiella michiganensis M5al)
MIPDVSQALAWLEKNPQALQGIQRGLERETLRVNADGTLATTGHPQALGSALTHKWITTD
FAEALLEFITPVDGDIQHMLTFMRDVHRYTARHIGDERMWPLSMPCYIAPGQDIELAQYG
TSNIGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGVEDAESGKEAISAGY
FRSIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPFQEASNGMYYLPYATSLRLSDLG
YTNKSQSNLGITFNDLYEYVSGLKRAIKTPSEEYENIGLEKDGKRLQINSNVLQIENELY
APIRPKRVTRSGETPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQVRFLDLFMVWCVLA
DAPEMSSDELLCTRTNWNRVILEGRKPGLTLGIGCETAQFPLAQVGKDLFRDLRRVAQTL
DSIHGCQAYQQACDELVACFDNPELTFSARILRSMLEEGIGGTGRELADRYRTMLREEPL
EILSEADFVAEREASLQRQEKVEAADSEPFEALLARHA